caa a22 4500 477034896 CHVBK 20180405111302.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/s002689900105 doi (NATIONALLICENCE)springer-10.1007/s002689900105 Functional and Morphometric Study of Cryopreserved Human Parathyroid Tissue Transplanted into Nude Mice [Elektronische Daten] [Yuji Tanaka, Hiroomi Funahashi, Tsuneo Imai, Masaki Wada, Yoshihiro Tominaga, Saroj K. Mishra, Hiroshi Takagi] Abstract. As part of an ongoing study of the characteristics of transplanted fresh normal and pathologic parathyroid tissues, we transplanted cryopreserved human parathyroid tissue into nude mice. Hyperplastic glands cryopreserved for various lengths of time (<2 weeks and >1 year) were transplanted into the gluteus muscle of nude mice. Specimens grafted were of two sizes: 10 mg and 30 mg. Serum human parathyroid hormone (hPTH) concentration was estimated by a double-antibody immunoradiometric assay prior to transplantation and 2, 4, and 8 weeks after transplantation. A low-calcium diet was given to some mice to evaluate any effects on the grafts of low serum concentrations of calcium. All mice were killed 4 hours after injection of bromodeoxyuridine (Brd U) for assessment of the cell proliferation in grafted parathyroid tissue. Although hPTH secretion of cryopreserved tissue was only half that of fresh tissue, the cryopreserved tissue released hPTH, as did fresh tissue. hPTH secretion was accelerated by stimulation of a low-calcium diet, and PTH secretion was positively correlated with the volume of transplanted tissue. The number of Brd U-immunoreactive cells was correlated with the serum concentration of hPTH (: r = 0.95), which indicates that cell proliferation is closely related to PTH secretion under the condition of successful transplantation. The experimental transplantation of cryopreserved tissue into nude mice, coupled with a concise immunohistochemical study of the grafts conducted prior to their transplantation into humans, can contribute to the evaluation of PTH secretion and cell proliferation. 1996 by the Société Internationale de Chir, ugie Tanaka Yuji Department of Surgery II, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan, JP aut Funahashi Hiroomi Department of Surgery II, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan, JP aut Imai Tsuneo Department of Surgery II, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan, JP aut Wada Masaki Department of Surgery II, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan, JP aut Tominaga Yoshihiro Department of Transplant Surgery, Nagoya Second Red Cross Hospital, Nagoya 466, Japan, JP aut Mishra Saroj K. Department of Endocrine Surgery, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India, IN aut Takagi Hiroshi Department of Surgery II, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan, JP aut https://doi.org/10.1007/s002689900105 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689900105 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanaka Yuji Department of Surgery II, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan, JP aut NATIONALLICENCE 700 1- Funahashi Hiroomi Department of Surgery II, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan, JP aut NATIONALLICENCE 700 1- Imai Tsuneo Department of Surgery II, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan, JP aut NATIONALLICENCE 700 1- Wada Masaki Department of Surgery II, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan, JP aut NATIONALLICENCE 700 1- Tominaga Yoshihiro Department of Transplant Surgery, Nagoya Second Red Cross Hospital, Nagoya 466, Japan, JP aut NATIONALLICENCE 700 1- Mishra Saroj K. Department of Endocrine Surgery, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India, IN aut NATIONALLICENCE 700 1- Takagi Hiroshi Department of Surgery II, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer