caa a22 4500 47703540X CHVBK 20180405111303.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/s002689900125 doi (NATIONALLICENCE)springer-10.1007/s002689900125 Effect of Growth Factors on Growth of Bovine Parathyroid Cells in Serum-Free Medium [Elektronische Daten] [Gregory P. Sadler, Derek L. Jones, J. Stuart Woodhead, Kieran Horgan, Malcolm H. Wheeler] Abstract. Published work has suggested a possible role of the epidermal growth factor receptor (EGFr) in parathyroid disease. Bovine parathyroid cells (BPCs) are frequently used as a tissue model for studying parathyroid disorders. We have studied the effect of the EGFr ligands EGF and transforming growth factor α (TGFα), alone and with insulin-like growth factors IGF-I and IGF-II on BPC growth. Experiments were run in triplicate and repeated three times. Cell numbers were assessed on day 5 by colorimetric MTT assay as well as by tritiated thymidine uptake. Results show that TGFα alone (: p < 0.05) and IGF-I and IGF-II alone ( p < 0.05) significantly stimulated growth over controls ( t -test). Furthermore, the combination of TGFα with IGF-I and IGF-II exhibited significant enhancement above that seen with IGF-I and IGF-II alone ( p < 0.01). EGF did not stimulate growth over controls. EGFr may be expressed in BPCs, but TGFα exhibits a more potent growth stimulus than EGF. Addition of IGF-I or IGF-II to the growth medium further enhances this effect. 1996 by the Société Internationale de Chir, ugie Sadler Gregory P. Department of Surgery, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, U.K., GB aut Jones Derek L. Department of Surgery, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, U.K., GB aut Woodhead J. Stuart Departments of Medical Biochemistry, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, U.K., GB aut Horgan Kieran Department of Surgery, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, U.K., GB aut Wheeler Malcolm H. Department of Surgery, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, U.K., GB aut https://doi.org/10.1007/s002689900125 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689900125 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sadler Gregory P. Department of Surgery, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, U.K., GB aut NATIONALLICENCE 700 1- Jones Derek L. Department of Surgery, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, U.K., GB aut NATIONALLICENCE 700 1- Woodhead J. Stuart Departments of Medical Biochemistry, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, U.K., GB aut NATIONALLICENCE 700 1- Horgan Kieran Department of Surgery, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, U.K., GB aut NATIONALLICENCE 700 1- Wheeler Malcolm H. Department of Surgery, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, U.K., GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer