caa a22 4500 477035450 CHVBK 20180405111304.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/s002689900141 doi (NATIONALLICENCE)springer-10.1007/s002689900141 Gip-2 Codon 179 Oncogene Mutations: Absent in Adrenal Cortical Tumors [Elektronische Daten] [Michael J. Demeure, Kara M. Doffek, Richard A. Komorowski, Jack Gorski] Abstract. The role of G protein mutations in the pathogenesis of adrenal cortex neoplasms is controversial. Two published studies disagree on the existence of a cysteine or histidine for arginine substitution at position 179 (R179C/H) of the GTP binding region of the α chain of an inhibitory G protein (Gi2α) in these tumors. Prior studies using detection by mutation-specific oligonucleotide hybridization showed either 3 of 11 or 0 of 56 tumors harbored mutations. To resolve this discrepancy and ascertain the importance of the R179C/H Gi2α mutation in the development of adrenal cortex tumors, we screened tumors from 29 patients (24 with adenoma, 5 with carcinoma) using a more sensitive assay employing polymerase chain reaction (PCR) and examination for restriction fragment length polymorphisms (RFLP). Detection of the potential R179C/H mutation by this technique was possible because the wild-type coding sequence includes the BSTU-1 restriction endonuclease recognition site CGCG, whereas the mutated gene does not. Results showed complete digestion of the amplified DNA samples from all 29 patients and the negative control DNA by BSTU-1, indicating that all tumor samples exhibited only the wild-type sequence. Direct sequencing of PCR product from four tumor samples confirmed the presence of only the wild-type sequence. The 0 of 29 rate of R179C/H mutations we found in Gi2α is different than the 3 of 11 positive rate (: p < 0.05, Fishers' exact) previously reported but agrees with the report showing 0 of 56 mutations. We conclude a mutation at position 179 of Gi 2 α is not important in the pathogenesis of most adrenal cortical tumors. 1996 by the Société Internationale de Chir, ugie Demeure Michael J. Department of Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, U.S.A., US aut Doffek Kara M. Department of Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, U.S.A., US aut Komorowski Richard A. Department of Pathology, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, U.S.A., US aut Gorski Jack Department of Cellular Biology and Anatomy, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, U.S.A., US aut https://doi.org/10.1007/s002689900141 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689900141 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Demeure Michael J. Department of Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, U.S.A., US aut NATIONALLICENCE 700 1- Doffek Kara M. Department of Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, U.S.A., US aut NATIONALLICENCE 700 1- Komorowski Richard A. Department of Pathology, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, U.S.A., US aut NATIONALLICENCE 700 1- Gorski Jack Department of Cellular Biology and Anatomy, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, U.S.A., US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer