caa a22 4500 477035477 CHVBK 20180405111304.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/s002689900059 doi (NATIONALLICENCE)springer-10.1007/s002689900059 Assessment of Proliferative Activity of Glandular Cells in Hyperfunctioning Parathyroid Gland Using Flow Cytometric and Immunohistochemical Methods [Elektronische Daten] [Pan Xiao-Lin, Naohiko Koide, Shinya Kobayashi, Masaru Kobayashi, Akira Sugenoya, Futoshi Iida, Tsutomu Katsuyama, Nobuteru Usuda, Tetsuji Nagata] Abstract. The proliferative activity of epithelial cells in hyperfunctioning parathyroid glands was estimated by flow cytometric and immunohistochemical procedures. A total of 30 parathyroid glands, 29 hyperfunctioning glands, and 1 normal gland were studied. The pathology of the 29 glands was determined to be hyperplasia in 19 and adenoma in 10. The S-phase cell population was expressed in terms of the S-phase fraction (%SPF) calculated from the histogram by DNA flow cytometry and in terms of the BrdU immunostaining labeling index (BrdU LI). Cells in all stages of the cell cycle were studied by Ki-67 immunostaining and expressed in terms of the labeling index (Ki-67 LI). Both BrdU LI and Ki-67 LI values were low, ranging from 0% to 0.58% and from 0.21% to 2.62%, respectively. The BrdU LIs were lower than the Ki-67 LIs, ranging from one-sixth to one-twelfth of the values depending on the disease. There were significant correlations between the two indices (: p < 0.001). The %SPF determined by flow cytometry was consistently higher than both the BrdU LI and the Ki-67 LI. This discrepancy cannot be explained precisely, and further improvements are required for the flow cytometric analysis of %SPF. The cell cycle study by BrdU and Ki-67 immunohistochemistry suggested that the glandular cells of the hyperfunctioning parathyroid were characterized by low proliferative activity. No evidence of rapid cell turnover rate assumed from the flow cytometric study could be observed in the hyperfunctioning parathyroid gland. 1996 by the Société Internationale de Chir, ugie Xiao-Lin Pan Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut Koide Naohiko Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut Kobayashi Shinya Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut Kobayashi Masaru Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut Sugenoya Akira Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut Iida Futoshi Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut Katsuyama Tsutomu Department of Clinical Laboratory, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut Usuda Nobuteru Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut Nagata Tetsuji Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut https://doi.org/10.1007/s002689900059 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689900059 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiao-Lin Pan Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut NATIONALLICENCE 700 1- Koide Naohiko Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut NATIONALLICENCE 700 1- Kobayashi Shinya Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut NATIONALLICENCE 700 1- Kobayashi Masaru Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut NATIONALLICENCE 700 1- Sugenoya Akira Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut NATIONALLICENCE 700 1- Iida Futoshi Department of Surgery, Shishu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut NATIONALLICENCE 700 1- Katsuyama Tsutomu Department of Clinical Laboratory, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut NATIONALLICENCE 700 1- Usuda Nobuteru Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut NATIONALLICENCE 700 1- Nagata Tetsuji Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer