caa a22 4500 477035957 CHVBK 20180405111305.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/s002689900161 doi (NATIONALLICENCE)springer-10.1007/s002689900161 Effects of Platelet-Activating Factor Antagonist E5880 on Intrahepatic and Systemic Metabolic Responses to Transient Hepatic Inflow Occlusion and Reperfusion in the Rabbit [Elektronische Daten] [Toshio Nakatani, Yoshimasa Sakamoto, Hirofumi Ando, Kunio Kobayashi] Abstract. We investigated the effects of pretreatment with a potent platelet-activating factor (PAF) receptor antagonist (E5880) on the changes in hepatic and systemic metabolism induced by transient hepatic ischemia and reperfusion. Sixty-five rabbits were assigned to four groups that either did or did not undergo a period of hepatic ischemia and reperfusion with or without pretreatment. E5880 was administered intraportally 1 minute prior to inflow occlusion. Twenty minutes of warm ischemia was followed by 30 minutes of reperfusion. Blood gas analyses and measurements of levels of arterial pyruvate, lactate, and ketone bodies, arterial and portal ammonia and endotoxin, and intrahepatic adenine nucleotide, pyruvate, and lactate were performed. Results were analyzed by either ANOVA or chi-square analysis. Hepatic tissue ATP and energy charge levels were significantly increased and the AMP level was significantly decreased after 30 minutes of reperfusion in the pretreatment group compared to those without pretreatment. At the same time, parameters reflecting hepatic mitochondrial function, such as the arterial ketone body ratio and arterial ammonia level, improved, although they were not statistically significant. No difference was observed for parameters reflecting systemic changes, such as arterial blood gas values and pyruvate and lactate levels. PAF is thought to mediate metabolic changes after hepatic ischemia and reperfusion. PAF released in the liver may exert local effects, which appear to be attenuated by pretreatment with E5880. Systemic metabolic changes seen after hepatic ischemia and reperfusion may be mediated by factors other than PAF.: 1996 by the Société Internationale de Chir, ugie Nakatani Toshio Trauma and Critical Center, Teikyo University School of Medicine, 11-1, Kaga 2-chome, Itabashi, Tokyo 173, Japan, JP aut Sakamoto Yoshimasa Division of Animal Care, Teikyo University School of Medicine, 11-1, Kaga 2-chome, Itabashi, Tokyo 173, Japan, JP aut Ando Hirofumi Division of Animal Care, Teikyo University School of Medicine, 11-1, Kaga 2-chome, Itabashi, Tokyo 173, Japan, JP aut Kobayashi Kunio Trauma and Critical Center, Teikyo University School of Medicine, 11-1, Kaga 2-chome, Itabashi, Tokyo 173, Japan, JP aut https://doi.org/10.1007/s002689900161 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689900161 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakatani Toshio Trauma and Critical Center, Teikyo University School of Medicine, 11-1, Kaga 2-chome, Itabashi, Tokyo 173, Japan, JP aut NATIONALLICENCE 700 1- Sakamoto Yoshimasa Division of Animal Care, Teikyo University School of Medicine, 11-1, Kaga 2-chome, Itabashi, Tokyo 173, Japan, JP aut NATIONALLICENCE 700 1- Ando Hirofumi Division of Animal Care, Teikyo University School of Medicine, 11-1, Kaga 2-chome, Itabashi, Tokyo 173, Japan, JP aut NATIONALLICENCE 700 1- Kobayashi Kunio Trauma and Critical Center, Teikyo University School of Medicine, 11-1, Kaga 2-chome, Itabashi, Tokyo 173, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer