caa a22 4500 477036082 CHVBK 20180405111305.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/s002689900162 doi (NATIONALLICENCE)springer-10.1007/s002689900162 Protective Effects of Antithrombin III Supplementation on Warm Ischemia and Reperfusion Injury in Rat Liver [Elektronische Daten] [Keiichi Okano, Yasutaka Kokudo, Kenji Okajima, Mohammad Akram Hossain, Ken Ishimura, Shinichi Yachida, Taijirou Tsubouchi, Hisao Wakabayashi, Takashi Maeba, Hajime Maeta] Abstract. The effect of antithrombin III (AT III) supplementation on energy status, microcirculation, cytoprotection, and prostacyclin (PGI2) production during and after a period of warm ischemia of the rat liver was investigated. AT III supplementation (250 units/kg) stimulate prostaglandin I2 (PGI2) production from 1 hour after administration, with maximal production observed at 3 hours. Ischemia was induced by occluding the hepatoduodenal ligament for 30 minutes, and experiments were continued for 60 minutes after reperfusion. The rats received AT III (250 units/kg IC) 30 minutes before induction of liver ischemia (AT III group). In the AT III group, recovery of the β-ATP/inorganic phosphate ratio measured by 31P nuclear magnetic resonance showed significant improvement (: p < 0.01), and the recovery of tissue blood flow markedly improved ( p < 0.01) compared to the saline-treated group (control group). Leakages of aspartame aminotransferase, alanine aminotransferase, and lactate dehydrogenase were mitigated in the AT III group ( p < 0.05). Ultrastructural alterations of sinusoidal endothelial cells were markedly reduced in the AT III group. The PGI 2 level at the end of reperfusion was significantly elevated ( p < 0.01) in the AT III group compared to the control group. The results of this study indicated that pretreatment with AT III significantly improved the energy status and microcirculation, as well as histologic damage, after liver ischemia and reperfusion. One of the fundamental effects of AT III might be mediated through the production of prostacyclin. 1996 by the Société Internationale de Chir, ugie Okano Keiichi First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut Kokudo Yasutaka First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut Okajima Kenji Department of Laboratory Medicine, Kumamoto University Medical School, 1-1-1 Honjo, Kumamoto 860, Japan, JP aut Hossain Mohammad Akram First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut Ishimura Ken First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut Yachida Shinichi First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut Tsubouchi Taijirou First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut Wakabayashi Hisao First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut Maeba Takashi First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut Maeta Hajime First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut https://doi.org/10.1007/s002689900162 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689900162 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okano Keiichi First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut NATIONALLICENCE 700 1- Kokudo Yasutaka First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut NATIONALLICENCE 700 1- Okajima Kenji Department of Laboratory Medicine, Kumamoto University Medical School, 1-1-1 Honjo, Kumamoto 860, Japan, JP aut NATIONALLICENCE 700 1- Hossain Mohammad Akram First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut NATIONALLICENCE 700 1- Ishimura Ken First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut NATIONALLICENCE 700 1- Yachida Shinichi First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut NATIONALLICENCE 700 1- Tsubouchi Taijirou First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut NATIONALLICENCE 700 1- Wakabayashi Hisao First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut NATIONALLICENCE 700 1- Maeba Takashi First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut NATIONALLICENCE 700 1- Maeta Hajime First Department of Surgery, Kagawa Medical School, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer