caa a22 4500 477036279 CHVBK 20180405111306.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/s002689900154 doi (NATIONALLICENCE)springer-10.1007/s002689900154 Parathyroid Hormone Content Distinguishes True Normal Parathyroids from Parathyroids of Patients with Primary Hyperparathyroidism [Elektronische Daten] [Collin J. Weber, John Russell, John T. Chryssochoos, Mary Hagler, William C. McGarity] Abstract. The purpose of this study was to clarify the pathophysiology of primary hyperparathyroidism by looking for differences between parathyroids from eucalcemic patients and patients with primary hyperparathyroidism (HPT) with respect to the following parameters: intracellular parathyroid content of parathyroid hormone (PTH) and parathyroid hormone messenger RNA (PTH mRNA); and serum PTH and calcium levels of patients and patient age. Coded samples of human parathyroid biopsies were assayed for PTH content with a C-terminal-specific radioimmunoassay. Total cellular RNA was extracted, and PTH mRNA was quantified by dot-blot analysis. These results were tabulated along with associated data on patient age, preoperative serum PTH, and preoperative calcium levels. The content of PTH was significantly higher in true normal (TN) parathyroids than in parathyroids from patients with hyperparathyroidism. PTH content of adenomas and hyperplasias were similar. PTH content of normal parathyroids biopsied from patients with parathyroid adenomas (NA) was statistically higher than that of adenomas but statistically lower than that of TN parathyroids. PTH mRNA and PTH content were correlated (: p < 0.001) for TN parathyroid glands; however, it was not true for glands (grossly normal or otherwise) in patients with HPT. Patient groups were similar with regard to mean patient age and intracellular PTH mRNA levels. Hypercalcemic patients were similar with regard to preoperative serum calcium and PTH levels. NA parathyroids, adenomas, and hyperplasias are different from TN parathyroids with regard to their PTH content. PTH mRNA was similar across all groups. The relation between intracellular PTH mRNA and PTH was significantly absent in patients with HPT compared with TN glands. Furthermore, we have found that PTH content of normal parathyroid in patients with adenoma is similar to that of hyperplastic and adenoma tissues. These data suggest that the PTH content of parathyroid tissues may be of use in differentiating normal from abnormal parathyroids. 1996 by the Société Internationale de Chir, ugie Weber Collin J. Department of Surgery, Emory University School of Medicine, Surgical Research, 1639 Pierce Drive, WMB 5105, Atlanta, Georgia 30308, U.S.A., US aut Russell John Department of Internal Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, U.S.A., US aut Chryssochoos John T. Department of Surgery, Emory University School of Medicine, Surgical Research, 1639 Pierce Drive, WMB 5105, Atlanta, Georgia 30308, U.S.A., US aut Hagler Mary Department of Surgery, Emory University School of Medicine, Surgical Research, 1639 Pierce Drive, WMB 5105, Atlanta, Georgia 30308, U.S.A., US aut McGarity William C. Department of Surgery, Emory University School of Medicine, Surgical Research, 1639 Pierce Drive, WMB 5105, Atlanta, Georgia 30308, U.S.A., US aut https://doi.org/10.1007/s002689900154 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689900154 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weber Collin J. Department of Surgery, Emory University School of Medicine, Surgical Research, 1639 Pierce Drive, WMB 5105, Atlanta, Georgia 30308, U.S.A., US aut NATIONALLICENCE 700 1- Russell John Department of Internal Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, U.S.A., US aut NATIONALLICENCE 700 1- Chryssochoos John T. Department of Surgery, Emory University School of Medicine, Surgical Research, 1639 Pierce Drive, WMB 5105, Atlanta, Georgia 30308, U.S.A., US aut NATIONALLICENCE 700 1- Hagler Mary Department of Surgery, Emory University School of Medicine, Surgical Research, 1639 Pierce Drive, WMB 5105, Atlanta, Georgia 30308, U.S.A., US aut NATIONALLICENCE 700 1- McGarity William C. Department of Surgery, Emory University School of Medicine, Surgical Research, 1639 Pierce Drive, WMB 5105, Atlanta, Georgia 30308, U.S.A., US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer