caa a22 4500 477036309 CHVBK 20180405111306.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/s002689900157 doi (NATIONALLICENCE)springer-10.1007/s002689900157 Resuscitation of Ischemically Damaged Pancreas by the Two-Layer (University of Wisconsin Solution/Perfluorochemical) Mild Hypothermic Storage Method [Elektronische Daten] [Shinichi Matsumoto, Yoshikazu Kuroda, Hirofumi Fujita, Yasuki Tanioka, Yongsik Kim, Tetsuya Sakai, Mitsuaki Hamano, Yasuyuki Suzuki, Yonson Ku, Yoichi Saitoh] Abstract. We have shown that 24-hour preservation by a two-layer [University of Wisconsin solution (UW)/perfluorochemical (PFC)] cold storage method at 4°C allowed tissue ATP synthesis and resuscitated canine pancreases subjected to 90 minutes of warm ischemia. The purpose of this study was to examine whether the two-layer (UW/PFC) mild hypothermic storage method at 20°C could shorten a preservation period for recovery of ischemically damaged pancreas and clarify changes of tissue adenine nucleotide metabolism and tissue perfusions. After 90 minutes of warm ischemia, canine pancreas grafts were preserved by the two-layer method and then autotransplanted. Tissue adenine nucleotide levels at the end of preservation and tissue perfusions after reperfusion were measured. Pancreas grafts subjected to 90 minutes of warm ischemia did not survive (0 of 5), without preservation. During a 5-hour preservation by the two-layer cold storage method the grafts did not synthesize enough ATP to repair damaged cell, although tissue perfusions were maintained after reperfusion. Consequently, ischemically damaged pancreases were not resuscitated (0 of 3). However, during 5-hour preservation by the two-layer mild hypothermic storage method, the grafts supplied enough ATP for processes that repair damaged cells, and tissue perfusions were maintained after reperfusion. As a result, ischemically damaged grafts were resuscitated (5 of 5). We conclude that 5-hour preservation by the two-layer mild hypothermic storage method accelerates ATP synthesis, which is essential for repairing damaged cells and protects the vascular microcirculation. This method can resuscitate ischemically damaged pancreas faster and holds promise for pancreas-kidney transplantation from cardiac arrest donors.: 1996 by the Société Internationale de Chir, ugie Matsumoto Shinichi First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut Kuroda Yoshikazu First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut Fujita Hirofumi First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut Tanioka Yasuki First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut Kim Yongsik First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut Sakai Tetsuya First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut Hamano Mitsuaki First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut Suzuki Yasuyuki First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut Ku Yonson First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut Saitoh Yoichi First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut https://doi.org/10.1007/s002689900157 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689900157 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsumoto Shinichi First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut NATIONALLICENCE 700 1- Kuroda Yoshikazu First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut NATIONALLICENCE 700 1- Fujita Hirofumi First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut NATIONALLICENCE 700 1- Tanioka Yasuki First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut NATIONALLICENCE 700 1- Kim Yongsik First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut NATIONALLICENCE 700 1- Sakai Tetsuya First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut NATIONALLICENCE 700 1- Hamano Mitsuaki First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut NATIONALLICENCE 700 1- Suzuki Yasuyuki First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut NATIONALLICENCE 700 1- Ku Yonson First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut NATIONALLICENCE 700 1- Saitoh Yoichi First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Kobe 650, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer