caa a22 4500 477036538 CHVBK 20180405111306.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/s002689900088 doi (NATIONALLICENCE)springer-10.1007/s002689900088 Disseminated Epithelial Tumor Cells in Bone Marrow of Patients with Esophageal Cancer: Detection and Prognostic Significance [Elektronische Daten] [Stefan Thorban, Jürgen Roder, Hjalmar Nekarda, Armin Funk, Klaus Pantel, J. Siewert] Minimal residual disease in patients with operable esophageal cancer is frequently missed by current noninvasive tumor staging. Here we applied an immunocytochemical cytokeratin assay that allows identification of individual esophageal carcinoma cells disseminated to bone marrow. Prior to therapy, bone marrow was aspirated from the upper iliac crest of 71 patients with esophageal cancer at various disease stages as well as an age-matched control group of 20 noncarcinoma patients. Tumor cells in cytologic bone marrow preparations were detected with monoclonal antibodies (mAbs) CK2, KL1, and A45-B/B3 to epithelial cytokeratins (CKs) using the alkaline phosphatase antialkaline phosphatase method. CK-positive cells were found in 14 (36.8%) of 38 cancer patients treated with curative intent and 16 (48.5%) of 33 patients with extended disease. The overall frequency of these cells was 1 per 4 × 105 to 82 per 4 × 105 mononuclear cells with no significant differences between patients at different tumor stages. After a short median follow-up of 9.5 months (3-24 months), 7 of 11 patients who underwent complete surgical resection but had tumor cells in bone marrow presented with tumor relapse compared to 2 of 19 corresponding patients without such cells (p < 0.01). It was concluded that although bone marrow is not a preferential site of overt metastasis of esophageal cancer, the frequent occurrence of isolated tumor cells at this distant site indicates that hematogenous dissemination of viable malignant cells occurs early in tumor progression. Société Internationale de Chirugie, 1996 Thorban Stefan Department of Surgery, Technical University of Munich, Ismaningerstrasse 22, 81675, Munich, Germany aut Roder Jürgen Department of Surgery, Technical University of Munich, Ismaningerstrasse 22, 81675, Munich, Germany aut Nekarda Hjalmar Department of Surgery, Technical University of Munich, Ismaningerstrasse 22, 81675, Munich, Germany aut Funk Armin Institute of Pathology and Pathologic Anatomy, Technical University of Munich, Trogerstrasse 7, 81675, Munich, Germany aut Pantel Klaus Institute of Immunology, Ludwig-Maximilians University, Goethestrasse 31, 80336, Munich, Germany aut Siewert J. Department of Surgery, Technical University of Munich, Ismaningerstrasse 22, 81675, Munich, Germany aut World Journal of Surgery Springer-Verlag 20/5(1996-06-01), 567-573 0364-2313 20:5<567 1996 20 268 https://doi.org/10.1007/s002689900088 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689900088 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thorban Stefan Department of Surgery, Technical University of Munich, Ismaningerstrasse 22, 81675, Munich, Germany aut NATIONALLICENCE 700 1- Roder Jürgen Department of Surgery, Technical University of Munich, Ismaningerstrasse 22, 81675, Munich, Germany aut NATIONALLICENCE 700 1- Nekarda Hjalmar Department of Surgery, Technical University of Munich, Ismaningerstrasse 22, 81675, Munich, Germany aut NATIONALLICENCE 700 1- Funk Armin Institute of Pathology and Pathologic Anatomy, Technical University of Munich, Trogerstrasse 7, 81675, Munich, Germany aut NATIONALLICENCE 700 1- Pantel Klaus Institute of Immunology, Ludwig-Maximilians University, Goethestrasse 31, 80336, Munich, Germany aut NATIONALLICENCE 700 1- Siewert J. Department of Surgery, Technical University of Munich, Ismaningerstrasse 22, 81675, Munich, Germany aut NATIONALLICENCE 773 0- World Journal of Surgery Springer-Verlag 20/5(1996-06-01), 567-573 0364-2313 20:5<567 1996 20 268 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer