caa a22 4500 477036546 CHVBK 20180405111306.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/s002689900084 doi (NATIONALLICENCE)springer-10.1007/s002689900084 Pancreaticobiliary Maljunction-Associated Pancreatitis: An Experimental Study on the Activation of Pancreatic Phospholipase A2 [Elektronische Daten] [Tetsuro Nakamura, Akira Okada, Jun Higaki, Hiromasa Tojo, Mitsuhiro Okamoto] Congenital dilatation of the bile duct (CDBD), or choledochal cyst, is often complicated by recurrent pancreatitis. Reflux of pancreatic juice into the bile duct through pancreaticobiliary maljunction (PBM), an anomaly commonly associated with CDBD, and ensuing activation of pancreatic enzymes could be involved in the pathophysiologic mechanism of recurrent pancreatitis. A study was undertaken to follow the time course of the activity of phospholipase A2 (PLA2) in animal models of PBM. The assay procedures for PLA2 were evaluated, as were the conditions for separating the active enzyme from its inactive proenzyme (pro-PLA2) by immunoblotting. A rat model was designed according to Block's method with some modifications. The kinetics of prophospholipase A2 (proPLA2) activation in bile was examined by measuring PLA2 activity and by immunoblotting using anti-rat pancreatic enzyme antibody after separating PLA2 from its zymogen under nonreducing conditions. Experimental animals were divided into three groups: group 1 (PBM group) in which bile and pancreatic juice were mixed with occlusion of the papilla; group 2, in which the papilla and hepatic hilus were occluded without mixing the two juices; and group 3, in which simple laparotomy was done. In group 1 animals, pro-PLA2 in bile was activated to its active form. In group 2 animals, where proPLA2 was predominant, there was only slight elevation of PLA2 activity in bile. In group 1 an immunohistologic study demonstrated localization of PLA2 around necrotic foci in the pancreatic parenchyma. These results suggest the involvement of activated PLA2 in the pathogenesis of choledochal cyst-associated pancreatitis. Société Internationale de Chirugie, 1996 Nakamura Tetsuro Department of Pediatric Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565, Osaka, Japan aut Okada Akira Department of Pediatric Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565, Osaka, Japan aut Higaki Jun Department of Pediatric Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565, Osaka, Japan aut Tojo Hiromasa Department of Molecular and Physiological Chemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565, Osaka, Japan aut Okamoto Mitsuhiro Department of Molecular and Physiological Chemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565, Osaka, Japan aut World Journal of Surgery Springer-Verlag 20/5(1996-06-01), 543-550 0364-2313 20:5<543 1996 20 268 https://doi.org/10.1007/s002689900084 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689900084 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Tetsuro Department of Pediatric Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 700 1- Okada Akira Department of Pediatric Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 700 1- Higaki Jun Department of Pediatric Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 700 1- Tojo Hiromasa Department of Molecular and Physiological Chemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 700 1- Okamoto Mitsuhiro Department of Molecular and Physiological Chemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 773 0- World Journal of Surgery Springer-Verlag 20/5(1996-06-01), 543-550 0364-2313 20:5<543 1996 20 268 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer