caa a22 4500 477037607 CHVBK 20180405111309.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00049486 doi (NATIONALLICENCE)springer-10.1007/BF00049486 Sacks Peter Head & Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, 10021, New York, N.Y., USA aut Cell, tissue and organ culture as in vitro models to study the biology of squamous cell carcinomas of the head and neck [Elektronische Daten] [Peter Sacks] In vitro models are currently being used to study head and neck squamous cell carcinoma (HNSCC). Several hundred HNSCC cell lines have been established by various investigators and used to study a broad spectrum of questions related to head and neck cancer. The head and neck model with respect to multistage carcinogenesis is now complete. Several techniques exist for the culture of normal epithelial cells from the upper aerodigestive tract (UADT). The biology of these UADT cells (oral cavity, oropharynx, hypopharynx and larynx) is being studied. Successful culture of premalignant lesions (dysplastic mucosa, leukoplakia, erythroplakia) has resulted in establishment of a limited number of premalignant cell lines and cell cultures. HPV infection of normal oral epithelial cells for immortalization (∼ premalignant cells) coupled with transformation with carcinogens (malignant cells) has established an experimental model for progression. Two in vivo models for oral carcinogenesis, the 7,12 dimethylbenz(a)anthracene-induced hamster cheek pouch model and the 4-nitroquinoline-N-oxide rat oral model, have been established in culture. Thus, multistage carcinogenesis models have been established from both human tissues and animal models and include cultures of normal, premalignant and malignant cells. Culture techniques for growing dissociated primary tumor cells for short term experimental analysis are being used. The culture of normal or tumor tissue as organ/explant cultures allows for the maintenance of normal cell-cell and cell-matrix interactions, but limits experimentation since these cultures cannot be propagated. Several three dimensional model systems are being used to obtain this histological complexity but allow for experimentation. The ability to culture normal, premalignant and malignant cells coupled with the use of a variety of culture techniques, should allow for the continued growth and experimentation in head and neck cancer research. Kluwer Academic Publishers, 1996 epithelial cells nationallicence squamous cell carcinoma nationallicence premalignant nationallicence cell culture nationallicence in vitro nationallicence Cancer and Metastasis Reviews Kluwer Academic Publishers; http://www.wkap.nl 15/1(1996-03-01), 27-51 0167-7659 15:1<27 1996 15 10555 https://doi.org/10.1007/BF00049486 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00049486 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Sacks Peter Head & Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, 10021, New York, N.Y., USA aut NATIONALLICENCE 773 0- Cancer and Metastasis Reviews Kluwer Academic Publishers; http://www.wkap.nl 15/1(1996-03-01), 27-51 0167-7659 15:1<27 1996 15 10555 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer