caa a22 4500 477037917 CHVBK 20180405111309.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00054016 doi (NATIONALLICENCE)springer-10.1007/BF00054016 Role of intermediate filaments in migration, invasion and metastasis [Elektronische Daten] [Mary Hendrix, Elisabeth Seftor, Yi-Wen Chu, Katrina Trevor, Richard Seftor] Summary: The expression of intermediate filament proteins is remarkably tissue-specific which suggests that the intermediate filament (IF) type(s) present in cells is somehow related to their biological function. However, in some cancers-particularly malignant melanoma and breast carcinoma, there is a strong indication that vimentin and keratin IFs are coexpressed, thus presenting as a dedifferentiated or interconverted (between epithelial and mesenchymal) phenotype. In this review, twoin vitro models are presented which recapitulate the interconverted phenotype in human melanoma and breast carcinoma, and allow, for the first time, unique observations to be made with respect to the role of IFs in cancer progression. These studies have provided direct evidence linking overexpression of keratin IFs in human melanoma with increased migratory and invasive activityin vitro, which can be down-regulated by substituting dominant-negative keratin mutants. Overexpression of vimentin IFs in the breast carcinoma model leads to augmentation of motility and invasivenessin vitro, which can be transiently down-regulated by treatment with antisense oligonucleotides to vimentin. Additional experimental evidence suggests that the mechanism(s) responsible for the differential expression of metastatic properties associated with the interconverted phenotype rest(s) in the unique interaction, either direct or indirect, of IFs with specific integrins interacting with the extracellular matrix. In this review, we discuss the observations derived from the human melanoma and breast carcinoma models to address the hypothesis that the ability to coexpress vimentin and keratins confers a selective advantage to tumor cells in their interpretation of and response to signaling cues from the extracellular matrix. The ramifications of these observations are discussed with respect to the patholophysiology of the respectivein situ tumors. Kluwer Academic Publishers, 1996 keratins nationallicence vimentin nationallicence intermediate filaments nationallicence cancer nationallicence invasion nationallicence metastasis nationallicence Hendrix Mary Department of Anatomy, College of Medicine, The University of Iowa, 52242-1109, Iowa City, Iowa, USA aut Seftor Elisabeth Department of Anatomy, College of Medicine, The University of Iowa, 52242-1109, Iowa City, Iowa, USA aut Chu Yi-Wen Institute of Biomedical Sciences, Academia Sinica, 11529, Taipei, Taiwan aut Trevor Katrina St. Luke's Medical Center, 53215, Milwaukee, WI, USA aut Seftor Richard Department of Anatomy, College of Medicine, The University of Iowa, 52242-1109, Iowa City, Iowa, USA aut Cancer and Metastasis Reviews Kluwer Academic Publishers; http://www.wkap.nl 15/4(1996-12-01), 507-525 0167-7659 15:4<507 1996 15 10555 https://doi.org/10.1007/BF00054016 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00054016 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hendrix Mary Department of Anatomy, College of Medicine, The University of Iowa, 52242-1109, Iowa City, Iowa, USA aut NATIONALLICENCE 700 1- Seftor Elisabeth Department of Anatomy, College of Medicine, The University of Iowa, 52242-1109, Iowa City, Iowa, USA aut NATIONALLICENCE 700 1- Chu Yi-Wen Institute of Biomedical Sciences, Academia Sinica, 11529, Taipei, Taiwan aut NATIONALLICENCE 700 1- Trevor Katrina St. Luke's Medical Center, 53215, Milwaukee, WI, USA aut NATIONALLICENCE 700 1- Seftor Richard Department of Anatomy, College of Medicine, The University of Iowa, 52242-1109, Iowa City, Iowa, USA aut NATIONALLICENCE 773 0- Cancer and Metastasis Reviews Kluwer Academic Publishers; http://www.wkap.nl 15/4(1996-12-01), 507-525 0167-7659 15:4<507 1996 15 10555 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer