caa a22 4500 477038182 CHVBK 20180405111310.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF02740747 doi (NATIONALLICENCE)springer-10.1007/BF02740747 De Blas Angel Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City, 5100 Rockhill Road, 64110-2499, Kansas City, MO aut Brain GABAA receptors studied with subunit-specific antibodies [Elektronische Daten] [Angel De Blas] Brain GABAA/benzodiazepine receptors are highly heterogeneous. This heterogeneity is largely derived from the existence of many pentameric combinations of at least 16 different subunits that are differentially expressed in various brain regions and cell types. This molecular heterogeneity leads to binding differences for various ligands, such as GABA agonists and antagonists, benzodiazepine agonists, antagonists, and inverse agonists, steroids, barbiturates, ethanol, and Cl− channel blockers. Different subunit composition also leads to heterogeneity in the properties of the Cl− channel (such as conductance and open time); the allosteric interactions among subunits; and signal transduction efficacy between ligand binding and Cl− channel opening. The study of recombinant receptors expressed in heterologous systems has been very useful for understanding the functional roles of the different GABAA receptor subunits and the relationships between subunit composition, ligand binding, and Cl− channel properties. Nevertheless, little is known about the complete subunit composition of the native GABAA receptors expressed in various brain regions and cell types. Several laboratories, including ours, are using subunit-specific antibodies for dissecting the heterogeneity and subunit composition of native (not reconstituted) brain GABAA receptors and for revealing the cellular and subcellular distribution of these subunits in the nervous system. These studies are also aimed at understanding the ligand-binding, transduction mechanisms, and channel properties of the various brain GABAA receptors in relation to synaptic mechanisms and brain function. These studies could be relevant for the discovery and design of new drugs that are selective for some GABAA receptors and that have fewer side effects. Humana Press Inc, 1996 GABAA receptor nationallicence benzodiazepine receptor nationallicence antibody nationallicence mRNA nationallicence structure nationallicence Molecular Neurobiology Humana Press 12/1(1996-02-01), 55-71 0893-7648 12:1<55 1996 12 12035 https://doi.org/10.1007/BF02740747 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02740747 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- De Blas Angel Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City, 5100 Rockhill Road, 64110-2499, Kansas City, MO aut NATIONALLICENCE 773 0- Molecular Neurobiology Humana Press 12/1(1996-02-01), 55-71 0893-7648 12:1<55 1996 12 12035 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer