caa a22 4500 477038379 CHVBK 20180405111310.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF02740623 doi (NATIONALLICENCE)springer-10.1007/BF02740623 Single-cell analysis of gene expression in the nervous system [Elektronische Daten] Measurements at the edge of chaos [Diane O'Dowd, Martin Smith] The characteristic functions of tissues and organs results from the integrated activity of individual cells. Nowhere is this more evident than in the nervous system, where the activities of single neurons communicating via electrical and chemical signals mediate complex functions, such as learning and memory. The past decade has seen an explosion in the identification of genes encoding proteins, such as voltage-gated channels and neurotransmitter receptors, responsible for neuronal excitability. These studies have highlighted the fact that even within a neuroanatomically defined region, the coexistence of multiple cell types makes it difficult, if not impossible, to correlate patterns of gene expression with function The recent development of techniques sensitive enough to, study gene expression at the single-cell level promises to break this bottleneck to our further understanding. Using examples taken from our own laboratories and the work of others, we review these techniques, their application, and discuss some of the difficulties associated with the interpretation of the data. Humana Press Inc, 1996 Expression profiling nationallicence single-cell RT-PCR nationallicence multiplex RT-PCR nationallicence gene expression nationallicence patch-clamp nationallicence O'Dowd Diane Department of Anatomy and Neurobiology, Developmental and Cell Biology, U.C. Irvine, 92697-1280, Irvine, CA aut Smith Martin Department of Anatomy and Neurobiology, Developmental and Cell Biology, U.C. Irvine, 92697-1280, Irvine, CA aut Molecular Neurobiology Humana Press 13/3(1996-12-01), 199-211 0893-7648 13:3<199 1996 13 12035 https://doi.org/10.1007/BF02740623 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02740623 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- O'Dowd Diane Department of Anatomy and Neurobiology, Developmental and Cell Biology, U.C. Irvine, 92697-1280, Irvine, CA aut NATIONALLICENCE 700 1- Smith Martin Department of Anatomy and Neurobiology, Developmental and Cell Biology, U.C. Irvine, 92697-1280, Irvine, CA aut NATIONALLICENCE 773 0- Molecular Neurobiology Humana Press 13/3(1996-12-01), 199-211 0893-7648 13:3<199 1996 13 12035 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer