caa a22 4500 477038646 CHVBK 20180405111311.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00985819 doi (NATIONALLICENCE)springer-10.1007/BF00985819 Genetic variation and natural hybridization between Orchis laxiflora and Orchis palustris ( Orchidaceae ) [Elektronische Daten] [Paola Arduino, Federica Verra, Rossella Cianchi, Walter Rossi, Bruno Corrias, Luciano Bullini] Genetic divergence between population samples ofOrchis laxiflora and ofO. palustris from various European locations was studied by electrophoretic analysis of 25 enzyme loci. An average genetic distance of DNei = 1.24 was found between the two taxa, with 12 out of 25 loci showing alternative alleles (diagnostic loci). Genetic heterogeneity was observed within bothO. laxiflora andO. palustris, when northern and southeastern populations were compared, being lower in the former taxon (D = 0.06), than in the latter (D = 0.16). Karyologically, 2n = 36 was found for bothO. laxiflora andO. palustris. O. laxiflora andO. palustris produce hybrids, described asO. ×intermedia. Genotype analysis of several sympatric samples showed the presence of hybrid zones, including F1 hybrids and, in low proportions, recombinant classes, putatively assigned to Fn and backcrosses, as well as a few introgressed individuals of both taxa. These data indicate that hybrids are only partially fertile, with a very limited mixing up of the two parental gene pools; this is also shown by the lack of significant lowering of genetic distances when sympatric and allopatric heterospecific samples are compared. Accordingly,O. laxiflora andO. palustris form a syngameon; nevertheless they can be considered as good taxonomic species, with virtually distinct gene pools, which evolve independently. The genetic variability inO. laxiflora andO. palustris is remarkably low ( $$\bar H$$ e = 0.05 and $$\bar H$$ e = 0.02, respectively). In particular, nearly complete absence of polymorphic loci was found inO. palustris from northcentral Europe. Two hypotheses are considered to explain the low genetic variability of this endangered species. Springer-Verlag, 1996 Orchidaceae nationallicence Orchis laxiflora nationallicence O. palustris nationallicence Isozymes nationallicence genetic structure nationallicence gene flow nationallicence genetic distance nationallicence chromosome number nationallicence hybridization nationallicence biochemical taxonomy nationallicence endangered species nationallicence Arduino Paola Dept. Genetics and Molecular Biology, Univ. Rome "La Sapienza”, Via Lancisi 29, I-00161, Rome, Italy aut Verra Federica Dept. Genetics and Molecular Biology, Univ. Rome "La Sapienza”, Via Lancisi 29, I-00161, Rome, Italy aut Cianchi Rossella Dept. Genetics and Molecular Biology, Univ. Rome "La Sapienza”, Via Lancisi 29, I-00161, Rome, Italy aut Rossi Walter Dept. Environmental Sciences, Univ. L'Aquila, Coppito, I-67010, L'Aquila, Italy aut Corrias Bruno Dept. Botany and Plant Ecology, Univ. Sassari, Via Muroni 25, I-07100, Sassari, Italy aut Bullini Luciano Dept. Genetics and Molecular Biology, Univ. Rome "La Sapienza”, Via Lancisi 29, I-00161, Rome, Italy aut Plant Systematics and Evolution Springer-Verlag 202/1-2(1996-03-01), 87-109 0378-2697 202:1-2<87 1996 202 606 https://doi.org/10.1007/BF00985819 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00985819 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arduino Paola Dept. Genetics and Molecular Biology, Univ. Rome "La Sapienza”, Via Lancisi 29, I-00161, Rome, Italy aut NATIONALLICENCE 700 1- Verra Federica Dept. Genetics and Molecular Biology, Univ. Rome "La Sapienza”, Via Lancisi 29, I-00161, Rome, Italy aut NATIONALLICENCE 700 1- Cianchi Rossella Dept. Genetics and Molecular Biology, Univ. Rome "La Sapienza”, Via Lancisi 29, I-00161, Rome, Italy aut NATIONALLICENCE 700 1- Rossi Walter Dept. Environmental Sciences, Univ. L'Aquila, Coppito, I-67010, L'Aquila, Italy aut NATIONALLICENCE 700 1- Corrias Bruno Dept. Botany and Plant Ecology, Univ. Sassari, Via Muroni 25, I-07100, Sassari, Italy aut NATIONALLICENCE 700 1- Bullini Luciano Dept. Genetics and Molecular Biology, Univ. Rome "La Sapienza”, Via Lancisi 29, I-00161, Rome, Italy aut NATIONALLICENCE 773 0- Plant Systematics and Evolution Springer-Verlag 202/1-2(1996-03-01), 87-109 0378-2697 202:1-2<87 1996 202 606 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer