caa a22 4500 477038670 CHVBK 20180405111311.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00985242 doi (NATIONALLICENCE)springer-10.1007/BF00985242 Chromosome banding and genome size differentiation in Prospero ( Hyacinthaceae ): Diploids [Elektronische Daten] [Irma Ebert, Johann Greilhuber, Franz Speta] Prospero is a Mediterranean autumn-flowering genus ofHyacinthaceae commonly classified inScilla asS. autumnalis andS. obtusifolia. Extensive dysploid and polyploid variation has been reported. In the present study 77 diploid accessions from the western to the eastern part of the area of distribution, the major part being from continental Greece and Crete, have been analysed for karyotype structure and, in part, for genome size. Methods employed were acetocarmine staining, Giemsa C-banding, fluorochrome staining mainly with chromomycin A3/DAPI, silver impregnation, and Feulgen densitometry. Banded idiograms were established with a computer assisted karyotype analysis procedure. Chromosome numbers were 2n = 8 inP. obtusifolium, and 2n = 12 and 14 inP. autumnale s. l. Dispensable euchromatic chromosome segments and different types of B chromosomes occurred. Among the cytotypes with 2n = 14 two karyotypes from Turkey differed from each other and from the rest in form, position of the nucleolar constriction, and in genome size. The remaining accessions were similar in karyotype shape but three levels of genome size could be discerned, the highest (1C = 7.50 pg) being found on the Iberian Peninsula, an intermediate one on Corsica and Malta, and the lowest (4.27 pg) in the Aegean. The karyotype with 2n = 12 had an intermediate genome size, and that ofP. obtusifolium a relatively low one. Heterochromatin amount was generally low, but some karyotypes showed characteristic banding patterns. The relationship between the chromosome complements with 2n = 14, 12 and 8 is discussed on the basis of idiograms and DNA amounts. Springer-Verlag, 1996 Hyacinthaceae nationallicence Prospero autumnale nationallicence P. obtusifolium nationallicence Scilla autumnalis nationallicence S. obtusifolia nationallicence Karyotype analysis nationallicence C-banding nationallicence fluorochrome banding nationallicence genome size nationallicence chromosome evolution nationallicence Mediterranean region nationallicence Ebert Irma Institute of Botany of the University of Vienna, Rennweg 14, A-1030, Wien, Austria aut Greilhuber Johann Institute of Botany of the University of Vienna, Rennweg 14, A-1030, Wien, Austria aut Speta Franz Biologiezentrum, Oberösterreichisches Landesmuseum, Johann-Wilhelm-Klein-Straße 73, A-4040, Linz, Austria aut Plant Systematics and Evolution Springer-Verlag 203/1-2(1996-03-01), 143-177 0378-2697 203:1-2<143 1996 203 606 https://doi.org/10.1007/BF00985242 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00985242 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ebert Irma Institute of Botany of the University of Vienna, Rennweg 14, A-1030, Wien, Austria aut NATIONALLICENCE 700 1- Greilhuber Johann Institute of Botany of the University of Vienna, Rennweg 14, A-1030, Wien, Austria aut NATIONALLICENCE 700 1- Speta Franz Biologiezentrum, Oberösterreichisches Landesmuseum, Johann-Wilhelm-Klein-Straße 73, A-4040, Linz, Austria aut NATIONALLICENCE 773 0- Plant Systematics and Evolution Springer-Verlag 203/1-2(1996-03-01), 143-177 0378-2697 203:1-2<143 1996 203 606 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer