caa a22 4500 477038778 CHVBK 20180405111311.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00985235 doi (NATIONALLICENCE)springer-10.1007/BF00985235 Nkongolo K. Department of Biological Sciences, Laurentian University, P3E-2C6, Sudbury, Ontario, Canada aut Chromosome analysis and DNA homology in three Picea species, P. mariana, P. rubens, and P. glauca ( Pinaceae ) [Elektronische Daten] [K. Nkongolo] A detailed karyotype analysis was made on the somatic complement ofPicea rubens andP. glauca. B-chromosomes were observed in someP. glauca populations. The karyotypes are generally asymmetrical with most of the chromosomes having median to median-submedian centromeres.Picea glauca chromosomes 2, 3, 7, and 8 have secondary constriction on their short arm and chromosome 10 has a secondary constriction on the long arm. Chromosome 3 was the most easily identifiable, as it has two secondary constrictions located on the short arm. InP. rubens, all the chromosomes but chromosomes 8 and 9 have one to four distinctive secondary constrictions. In general, the diagrammatic comparisons show a high degree of similarity amongP. mariana, P. rubens, andP. glauca. GenomicP. mariana probe strongly hybridized to dots of genomic DNA fromP. rubens andP. glauca indicating that there is a high sequence homology among these three species. The synchronizing agent, hydroxyurea was used at different concentrations to enhance the mitotic index of cell suspensions derived from embryogenic cultures. Hydroxyurea at 1.25 mM increased significantly the mitotic index. An increase of hydroxyurea from 1.25 mM to 5 mM and 10 mM resulted in a steady decrease of mitotic index. Springer-Verlag, 1996 Pinaceae nationallicence Picea nationallicence Spruce nationallicence mitotic index nationallicence cytotaxonomy nationallicence karyotype nationallicence DNA homology nationallicence Plant Systematics and Evolution Springer-Verlag 203/1-2(1996-03-01), 27-40 0378-2697 203:1-2<27 1996 203 606 https://doi.org/10.1007/BF00985235 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00985235 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Nkongolo K. Department of Biological Sciences, Laurentian University, P3E-2C6, Sudbury, Ontario, Canada aut NATIONALLICENCE 773 0- Plant Systematics and Evolution Springer-Verlag 203/1-2(1996-03-01), 27-40 0378-2697 203:1-2<27 1996 203 606 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer