caa a22 4500 477039189 CHVBK 20180405111312.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00985914 doi (NATIONALLICENCE)springer-10.1007/BF00985914 Low levels of genetic variation in Phenakospermum guyannense ( Strelitziaceae ), a widespread bat-pollinated Amazonian herb [Elektronische Daten] [Cheryl Roesel, W. Kress, Brunella Bowditch] Phenakospermum guyannense is a monotypic, arborescent, long-lived monocot that is widespread in Amazonian South America. This outcrossing species is pollinated primarily by phyllostomid bats. Given these life-history characteristics,P. guyannense is expected to exhibit high levels of genetic variation and gene flow. We used isozyme electrophoresis and randomly amplified polymorphic DNA (RAPD) to characterize genetic variation in populations ofP. guyannense from French Guiana. Both measures detected a surprisingly low level of genetic variation, with only five out of twenty (25%) allozyme loci polymorphic (P), 1.35 alleles per locus (A), and an expected heterozygosity (He) of 0.090 at the species level. Isozymic genetic variation was even lower within populations (P = 17.5, A = 1.24, He = 0.074), and was corroborated by a RAPD assay that used 26 arbitrary primers (P = 3.61, A = 1.04, He = 0.014). Although overall levels of variation were low, the detectable variation was distributed as would be expected for an outcrossing species with extensive gene flow (mean GST = 0.230). We suspect thatP. guyannense is depauperate in genetic variation because of a series of bottlenecks that affected the species over this portion of its range. Springer-Verlag, 1996 Strelitziaceae nationallicence Phenakospermum nationallicence Isozyme electrophoresis nationallicence RAPD nationallicence genetic variation nationallicence pollination nationallicence Roesel Cheryl Department of Botany, MRC-166, National Museum of Natural History, Smithsonian Institution, 20560, Washington, D.C., USA aut Kress W. Department of Botany, MRC-166, National Museum of Natural History, Smithsonian Institution, 20560, Washington, D.C., USA aut Bowditch Brunella Laboratory of Molecular Systematics, National Museum of Natural History, Smithsonian Institution, 20560, Washington, D.C., USA aut Plant Systematics and Evolution Springer-Verlag 199/1-2(1996-03-01), 1-15 0378-2697 199:1-2<1 1996 199 606 https://doi.org/10.1007/BF00985914 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00985914 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Roesel Cheryl Department of Botany, MRC-166, National Museum of Natural History, Smithsonian Institution, 20560, Washington, D.C., USA aut NATIONALLICENCE 700 1- Kress W. Department of Botany, MRC-166, National Museum of Natural History, Smithsonian Institution, 20560, Washington, D.C., USA aut NATIONALLICENCE 700 1- Bowditch Brunella Laboratory of Molecular Systematics, National Museum of Natural History, Smithsonian Institution, 20560, Washington, D.C., USA aut NATIONALLICENCE 773 0- Plant Systematics and Evolution Springer-Verlag 199/1-2(1996-03-01), 1-15 0378-2697 199:1-2<1 1996 199 606 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer