caa a22 4500 477039421 CHVBK 20180405111313.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00989052 doi (NATIONALLICENCE)springer-10.1007/BF00989052 The karyotype of Festucopsis serpentini ( Poaceae Triticeae ) from Albania studied by banding techniques and in situ hybridization [Elektronische Daten] [Ib Linde-Laursen, Ole Seberg, Signe Frederiksen, Claus Baden] The karyotypes of two populations ofFestucopsis serpentini (2n = 2x = 14) endemic to Albania were investigated in detail by Giemsa C- and N-banding, AgNO3 staining, and in situ hybridization with an rDNA probe. The complements consisted of 14 large chromosomes, 10 metacentric and 4 SAT-chromosomes, a metacentric and a submetacentric pair. SAT-chromosomes from one population carried exclusively minute satellites, whereas SAT-chromosomes from another population also carried larger polymorphic satellites, suggesting a geographical differentiation. The existence of four chromosomes with nucleolus forming activity was established through AgNO3 staining; however, the rDNA probe additionally hybridized to intercalary positions in the short arms of two metacentric chromosomes revealing two inactive rDNA sites. C-banding patterns comprised from zero and up to four very small to larger, generally telomeric bands per chromosome giving low levels of constitutive heterochromatin. Similarities in chromosome morphology and C-banding patterns identified the homologous relationships of all chromosomes in one population, but of three pairs only in the other. Reliable identification of homologous chromosomes between plants was only possible for the SAT-chromosomes. A comparison between the C-banded karyotypes ofF. serpentini andPeridictyon sanctum supports their position in two genera. Springer-Verlag, 1996 Poaceae nationallicence Triticeae nationallicence Festucopsis nationallicence Peridictyon nationallicence Giemsa nationallicence C-banding nationallicence N-banding nationallicence silver staining nationallicence in situ hybridization nationallicence FISH nationallicence karyotype nationallicence chromosome polymorphism nationallicence nucleoli nationallicence rDNA nationallicence Linde-Laursen Ib Environmental Science and Technology Department, Plant Genetics, Risø National Laboratory, DK-4000, Roskilde, Denmark aut Seberg Ole Botanical Institute, Botanical Laboratory, University of Copenhagen, Gothersgade 140, DK-1123, Copenhagen K, Denmark aut Frederiksen Signe Botanical Institute, Botanical Laboratory, University of Copenhagen, Gothersgade 140, DK-1123, Copenhagen K, Denmark aut Baden Claus Botanical Section, The Royal Veterinary and Agricultural University, Rolighedsvej 23, DK-1958, Frederiksberg C (Copenhagen), Denmark aut Plant Systematics and Evolution Springer-Verlag 201/1-4(1996-03-01), 75-82 0378-2697 201:1-4<75 1996 201 606 https://doi.org/10.1007/BF00989052 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00989052 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Linde-Laursen Ib Environmental Science and Technology Department, Plant Genetics, Risø National Laboratory, DK-4000, Roskilde, Denmark aut NATIONALLICENCE 700 1- Seberg Ole Botanical Institute, Botanical Laboratory, University of Copenhagen, Gothersgade 140, DK-1123, Copenhagen K, Denmark aut NATIONALLICENCE 700 1- Frederiksen Signe Botanical Institute, Botanical Laboratory, University of Copenhagen, Gothersgade 140, DK-1123, Copenhagen K, Denmark aut NATIONALLICENCE 700 1- Baden Claus Botanical Section, The Royal Veterinary and Agricultural University, Rolighedsvej 23, DK-1958, Frederiksberg C (Copenhagen), Denmark aut NATIONALLICENCE 773 0- Plant Systematics and Evolution Springer-Verlag 201/1-4(1996-03-01), 75-82 0378-2697 201:1-4<75 1996 201 606 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer