caa a22 4500 477046630 CHVBK 20180405111332.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02861451 doi (NATIONALLICENCE)springer-10.1007/BF02861451 Ecogeographic distribution of Phaseolus spp. (Fabaceae) in Bolivia [Elektronische Daten] [Rosanna Freyre, Raúl Ríos, Lorena Guzmán, Daniel Debouck, Paul Gepts] Distributión Ecogeográfica de Phaseolus Sp. (Fabaceae) en Bolivia WildPhaseolus vulgaris is distributed between northern Mexico and northern Argentina. Analysis of phaseolin and molecular markers (isozymes, Restriction Fragment Length Polymorphisms or RFLPs) indicate that this gene pool consists of two major groups, Mesoamerican and Andean, and a third intermediate group found in northwestern South America. Previous to this study, only four accessions of wildP. vulgaris beans from Bolivia had been collected and their genetic relationship with other wild beans from Latin America was not known. Due to the problem of intense erosion in some areas of Bolivia, it was our objective to survey and documentPhaseolus spp. in this area before their extinction. We conducted a collection expedition in May 1994 in the departments of Cochabamba, Chuquisaca and Tarija. This resulted in collections of four populations ofP. augusti, two of cultivatedP. lunatus and two mixtures of cultivatedP. vulgaris. The first mixture was made of "k'opurus” or beans consumed after toasting, and represented an addition of 17 accessions to the Bolivian collection. The second mixture was made of "porotos” and resulted in the addition of 10 new accessions. Seven germplasm collections of wildP. vulgaris were found, which allowed us to increase the number of known populations of wild common bean for Bolivia. Another accession was found as a wild-weed-crop complex. Seven of these wildP. vulgaris accessions along with another accession from Bolivia collected previously, and a number of P. vulgaris accessions from Mexico (17), Guatemala (3), Colombia (10), Ecuador (6), Peru (17) and Argentina (16) were analyzed with RAPDs. The use of 14 random primers and one SCAR (Sequence Characterized Amplified Region) resulted in 90 bands, of which 83 were polymorphic. This data was used to construct a dendrogram which shows clear separation into three clusters, corresponding to each of the gene pools and an intermediate group. The Bolivian wild P. vulgaris beans grouped with the accessions of southern Peru and Argentina into the Andean gene pool. RAPD analysis of genetic diversity correlated well with genetic diversity obtained with other markers. Moreover, the ease of analysis allowed us to obtain a large number of bands which was conducive to greater sensitivity and identification of geographic subgroups and accessions of hybrid origin. The New York Botanical Garden, 1996 common bean nationallicence Phaseolus vulgaris nationallicence germplasm exploration nationallicence RAPDs nationallicence Freyre Rosanna Department of Agronomy and Range Science, University of California, 95616-8515, Davis, CA aut Ríos Raúl Centro de Investigaciones Fitogenérticas de Pairumani, Bolivia, Cochabamba aut Guzmán Lorena Centro de Investigaciones Fitogenérticas de Pairumani, Bolivia, Cochabamba aut Debouck Daniel CIAT, Apartado Aéreo 6713, Cali, Colombia aut Gepts Paul Department of Agronomy and Range Science, University of California, 95616-8515, Davis, CA aut Economic Botany Springer-Verlag 50/2(1996-04-01), 195-215 0013-0001 50:2<195 1996 50 12231 https://doi.org/10.1007/BF02861451 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02861451 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Freyre Rosanna Department of Agronomy and Range Science, University of California, 95616-8515, Davis, CA aut NATIONALLICENCE 700 1- Ríos Raúl Centro de Investigaciones Fitogenérticas de Pairumani, Bolivia, Cochabamba aut NATIONALLICENCE 700 1- Guzmán Lorena Centro de Investigaciones Fitogenérticas de Pairumani, Bolivia, Cochabamba aut NATIONALLICENCE 700 1- Debouck Daniel CIAT, Apartado Aéreo 6713, Cali, Colombia aut NATIONALLICENCE 700 1- Gepts Paul Department of Agronomy and Range Science, University of California, 95616-8515, Davis, CA aut NATIONALLICENCE 773 0- Economic Botany Springer-Verlag 50/2(1996-04-01), 195-215 0013-0001 50:2<195 1996 50 12231 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer