caa a22 4500 477052800 CHVBK 20180405111342.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00230363 doi (NATIONALLICENCE)springer-10.1007/BF00230363 Phosphorylation of style S-RNases by Ca2+-dependent protein kinases from pollen tubes [Elektronische Daten] [Caroline Kunz, Alan Chang, Jean-Denis Faure, Adrienne Clarke, Gideon Polya, Marilyn Anderson] Solanaceous plants with gametophytic self-incompatibility produce ribonucleases in the transmitting tract of the style that interact with self-pollen and inhibit its growth. These ribonucleases are a series of allelic products of the S-locus, which controls self-incompatibility. Little is known about the pollen components involved in this interaction or whether a signal transduction pathway is activated during the self-incompatibility response. We have partially purified a soluble protein kinase from pollen tubes of Nicotiana alata that phosphorylates the self-incompatibility RNases (S-RNases) from N. alata but not Lycopersicon peruvianum. The soluble protein kinase (Nak-1) has several features shared by the calcium-dependent protein kinase (CDPK) class of plant protein kinases, including substrate specificity, calcium dependence, inhibition by the calmodulin antagonist calmidazolium, and cross-reaction with monoclonal antibodies raised to a CDPK from soybean. Phosphorylation of S 2-RNase by Nak-1 is restricted to serine residues, but the site(s) of phosphorylation has not been determined and there is no evidence for allele-specific phosphorylation. The microsomal fraction from pollen tubes also phosphorylates S-RNases and this activity may be associated with proteins of Mr∼60 K and 69 K that cross-react with the monoclonal antibody to the soybean CDPK. These results are discussed in the context of the involvement of phosphorylation in other self-incompatibility systems. Springer-Verlag, 1996 Self-incompatibility nationallicence S -ribonucleases nationallicence Pollen nationallicence Protein kinases nationallicence Phosphorylation nationallicence Kunz Caroline Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut Chang Alan Department of Biochemistry, La Trobe University, 3083, Bundoora, Victoria, Australia aut Faure Jean-Denis Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut Clarke Adrienne Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut Polya Gideon Department of Biochemistry, La Trobe University, 3083, Bundoora, Victoria, Australia aut Anderson Marilyn Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut Sexual Plant Reproduction Springer-Verlag 9/1(1996-01-01), 25-34 0934-0882 9:1<25 1996 9 497 https://doi.org/10.1007/BF00230363 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00230363 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kunz Caroline Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Chang Alan Department of Biochemistry, La Trobe University, 3083, Bundoora, Victoria, Australia aut NATIONALLICENCE 700 1- Faure Jean-Denis Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Clarke Adrienne Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Polya Gideon Department of Biochemistry, La Trobe University, 3083, Bundoora, Victoria, Australia aut NATIONALLICENCE 700 1- Anderson Marilyn Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/1(1996-01-01), 25-34 0934-0882 9:1<25 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer