caa a22 4500 477052916 CHVBK 20180405111342.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00230364 doi (NATIONALLICENCE)springer-10.1007/BF00230364 Nuclear male sterility induced by pollen-specific expression of a ribonuclease [Elektronische Daten] [Xiao-yan Zhan, Hen-ming Wu, Alice Cheung] The promoter of a rice pollen-specific gene, PS1, has been fused to the Bacillus amyloliquefaciens Barnase gene which encodes a secreted ribonuclease. The PS1-Barnase chimeric gene has been introduced into tobacco. These transgenic tobacco plants show normal vegetative and floral development, but they display a range of reproductive properties from slightly reduced in fertility to completely sterile. Barnase mRNAs are detectable in the pollen from transgenic plants which do not show an obvious fertility-related phenotype, and in a few plants which have a mildly reduced-fertile phenotype. However, transgenic plants with a severely reduced-fertile or sterile phenotype do not accumulate detectable amounts of Barnase mRNA in their pollen, and the quality of their RNA is poor, presumably because of extensive RNA degradation. Reciprocal crosses between these transgenic plants and wild-type controls showed that the reduced-fertile phenotype is associated only with the transgenic pollen. When used as the female parent, these PS1-Barnase transgenic plants are fully fertile. Anthers in the severely sterile transgenic plants develop normally, but the majority of their pollen grains have abnormal morphology and they fail to germinate. These results indicate that expression of a pollen-specific cytotoxic gene induces lethality in pollen and may lead to severely reduced male fertility. Springer-Verlag, 1996 Barnase nationallicence Engineered male sterility nationallicence Pollen-specific expression nationallicence Transgenic plants nationallicence Zhan Xiao-yan Department of Biology, Yale University, P.O. Box 20-8104, 06520-8104, New Haven, CT, USA aut Wu Hen-ming Department of Biology, Yale University, P.O. Box 20-8104, 06520-8104, New Haven, CT, USA aut Cheung Alice Department of Biology, Yale University, P.O. Box 20-8104, 06520-8104, New Haven, CT, USA aut Sexual Plant Reproduction Springer-Verlag 9/1(1996-01-01), 35-43 0934-0882 9:1<35 1996 9 497 https://doi.org/10.1007/BF00230364 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00230364 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhan Xiao-yan Department of Biology, Yale University, P.O. Box 20-8104, 06520-8104, New Haven, CT, USA aut NATIONALLICENCE 700 1- Wu Hen-ming Department of Biology, Yale University, P.O. Box 20-8104, 06520-8104, New Haven, CT, USA aut NATIONALLICENCE 700 1- Cheung Alice Department of Biology, Yale University, P.O. Box 20-8104, 06520-8104, New Haven, CT, USA aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/1(1996-01-01), 35-43 0934-0882 9:1<35 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer