caa a22 4500 47705319X CHVBK 20180405111342.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF02221392 doi (NATIONALLICENCE)springer-10.1007/BF02221392 Molecular characterization of profilin isoforms from tobacco ( Nicotiana tabacum ) pollen [Elektronische Daten] [Irene Mittermann, Susanne Heiss, Dietrich Kraft, Rudolf Valenta, Erwin Heberle-Bors] Profilins are actin-binding proteins in eukaryotes which participate in the phosphoinositide pathway via binding to PIP2. Using polyclonal rabbit sera raised against plant profilins, the occurrence of several profilin isoforms is demonstrated in two-dimensionally analyzed tobacco pollen extracts. The cDNAs coding for two novel tobacco profilin isoforms (ntPro2, ntPro3) were isolated from a pollen cDNA library by antibody screening. When the cDNA and deduced amino acid sequences of the two isoforms were compared with a previously isolated tobacco pollen profilin cl)NA (ntPro1), significant differences were noted in the non-coding regions, whereas the coding sequences, in particular the functional domains, showed little variation. The cDNAs coding for the three tobacco profilin isoforms were expressed inEscherichia coli and shown to bind comparably to different anti-profilin antisera. The high degree of similarity among the different tobacco pollen profilin isoforms points to functional equivalence. Assuming that the presence of profilin is indispensable to the control of the large amounts of actin present in pollen, the occurrence of different profilin isoforms in pollen is interpreted to represent a protective mechanism against loss of profilin functions. Springer-Verlag, 1996 Profilin nationallicence Tobacco pollen nationallicence Nicotiana tabacum nationallicence cDNA cloning nationallicence Isoforms nationallicence Mittermann Irene Institute of General and Experimental Pathology, AKH, University of Vienna, Währingergürtel 18-20, A-1090, Vienna, Austria aut Heiss Susanne Institute of General and Experimental Pathology, AKH, University of Vienna, Währingergürtel 18-20, A-1090, Vienna, Austria aut Kraft Dietrich Institute of General and Experimental Pathology, AKH, University of Vienna, Währingergürtel 18-20, A-1090, Vienna, Austria aut Valenta Rudolf Institute of General and Experimental Pathology, AKH, University of Vienna, Währingergürtel 18-20, A-1090, Vienna, Austria aut Heberle-Bors Erwin Institute of Microbiology and Genetics, Vienna Biocenter, University of Vienna, Vienna, Austria aut Sexual Plant Reproduction Springer-Verlag 9/3(1996-05-01), 133-139 0934-0882 9:3<133 1996 9 497 https://doi.org/10.1007/BF02221392 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02221392 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mittermann Irene Institute of General and Experimental Pathology, AKH, University of Vienna, Währingergürtel 18-20, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Heiss Susanne Institute of General and Experimental Pathology, AKH, University of Vienna, Währingergürtel 18-20, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Kraft Dietrich Institute of General and Experimental Pathology, AKH, University of Vienna, Währingergürtel 18-20, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Valenta Rudolf Institute of General and Experimental Pathology, AKH, University of Vienna, Währingergürtel 18-20, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Heberle-Bors Erwin Institute of Microbiology and Genetics, Vienna Biocenter, University of Vienna, Vienna, Austria aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/3(1996-05-01), 133-139 0934-0882 9:3<133 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer