caa a22 4500 477053343 CHVBK 20180405111343.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF02221399 doi (NATIONALLICENCE)springer-10.1007/BF02221399 Identification of genes expressed in the shoot apex of Brassica campestris during floral transition [Elektronische Daten] [Hiroyasu Kitashiba, Takayoshi Iwai, Kinya Toriyama, Masao Watanabe, Kokichi Hinata] The vegetative-to-floral transition ofBrassica campestris cv. Osome was induced by vernalization. Poly(A)+RNA was isolated from the transition shoot apex after 6 weeks of vernalization, the floral apex after 12 weeks of vernalization and the expanded leaves just before vernalization, and cDNAs were synthesized. These cDNAs were used for subtraction and differential screening to select cDNA preferentially present in the transition and floral apices. Nucleotide sequences of the resulting 14 cDNA clones were determined, and northern blot analysis was carried out on six cDNAs. Two cDNA clones which did not show significant similarity to known genes were shown to be preferentially expressed in the floral apex. Springer-Verlag, 1996 Brassica campestris nationallicence cDNA cloning nationallicence Floral transition nationallicence Shoot apex nationallicence Vernalization nationallicence Kitashiba Hiroyasu Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, 981, Aobaku, Sendai, Japan aut Iwai Takayoshi Miyagi Prefecture Agricultural Research Center, Takadate, Natori, 981-12, Miyagi, Japan aut Toriyama Kinya Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, 981, Aobaku, Sendai, Japan aut Watanabe Masao Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, 981, Aobaku, Sendai, Japan aut Hinata Kokichi Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, 981, Aobaku, Sendai, Japan aut Sexual Plant Reproduction Springer-Verlag 9/3(1996-05-01), 186-188 0934-0882 9:3<186 1996 9 497 https://doi.org/10.1007/BF02221399 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02221399 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kitashiba Hiroyasu Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, 981, Aobaku, Sendai, Japan aut NATIONALLICENCE 700 1- Iwai Takayoshi Miyagi Prefecture Agricultural Research Center, Takadate, Natori, 981-12, Miyagi, Japan aut NATIONALLICENCE 700 1- Toriyama Kinya Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, 981, Aobaku, Sendai, Japan aut NATIONALLICENCE 700 1- Watanabe Masao Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, 981, Aobaku, Sendai, Japan aut NATIONALLICENCE 700 1- Hinata Kokichi Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, 981, Aobaku, Sendai, Japan aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/3(1996-05-01), 186-188 0934-0882 9:3<186 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer