caa a22 4500 47705336X CHVBK 20180405111343.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02173099 doi (NATIONALLICENCE)springer-10.1007/BF02173099 SLR1 function is dispensable for both self-incompatible rejection and self-compatible pollination processes in Brassica [Elektronische Daten] [T. Franklin, J. Oldknow, M. Trick] TheSLR1 gene inBrassica is related both in DNA sequence and in pattern of expression to theS-locus glycoprotein (SLG) gene involved in the self-incompatibility mechanism which recognises and arrests the germination of self pollen. However,SLR1 shows minimal allelic variation and is expressed in both self-incompatible and compatibleBrassica lines and in related, self-compatible cruciferous plants. The function of the SLR1 protein is unknown. TheSLR1 gene was specifically ablated in self-incompatible and self-compatibleBrassica plants byAgrobacterium-mediated transformation with an antisense construct. Primary transformants and homozygous T2 progeny of both self-incompatibleB. oleracea and self-compatibleB. napus recipients were found to exhibit normal pollination responses despite having no detectable SLR1 glycoprotein. This shows that the high, wild-type level of SLR1 protein is not required to sustain the self-incompatibility reaction, nor is it necessary for successful intra-specific cross-pollination between compatible lines. Springer-Verlag, 1996 Brassica nationallicence Self-incompatibility nationallicence SLR1 Transformation nationallicence Antisense nationallicence Franklin T. Department of Brassica and Oilseeds Research, John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, UK aut Oldknow J. Department of Brassica and Oilseeds Research, John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, UK aut Trick M. Department of Brassica and Oilseeds Research, John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, UK aut Sexual Plant Reproduction Springer-Verlag 9/4(1996-07-01), 203-208 0934-0882 9:4<203 1996 9 497 https://doi.org/10.1007/BF02173099 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02173099 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Franklin T. Department of Brassica and Oilseeds Research, John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, UK aut NATIONALLICENCE 700 1- Oldknow J. Department of Brassica and Oilseeds Research, John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, UK aut NATIONALLICENCE 700 1- Trick M. Department of Brassica and Oilseeds Research, John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, UK aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/4(1996-07-01), 203-208 0934-0882 9:4<203 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer