caa a22 4500 477053386 CHVBK 20180405111343.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02173104 doi (NATIONALLICENCE)springer-10.1007/BF02173104 Immunoelectron microscopy of myosin associated with the generative cells in pollen tubes of Nicotiana tabacum L [Elektronische Daten] [Uday Tirlapur, Claudia Faleri, Mauro Cresti] In this study, polyclonal anti-myosin antibodies were used for immunogold labeling of ultrathin sections of pollen tubes ofNicotiana tabacum L. to unravel the ultrastructural localization of myosin associated with the generative cells. Clusters of immunogold particles were consistently found in association with the area of the outer surface of the vegetative cell plasma membrane present around the generative cell. Compared to the generative cell cytoplasm, the nucleoplasm showed higher numbers of gold particles. This is the first direct evidence demonstrating the presence of myosin in the nuclei of the generative cell of flowering plants. The possible implications of these findings are discussed in relation to movement of the generative cell in the pollen tube cytoplasm. Springer-Verlag, 1996 Generative cell nationallicence Nicotiana tabacum nationallicence Immunogold nationallicence Myosin nationallicence Pollen tube nationallicence Tirlapur Uday Dipartimento di Biologia Ambientale, Universita di Siena, Via P.A. Mattioli 4, I-53100, Siena, Italy aut Faleri Claudia Dipartimento di Biologia Ambientale, Universita di Siena, Via P.A. Mattioli 4, I-53100, Siena, Italy aut Cresti Mauro Dipartimento di Biologia Ambientale, Universita di Siena, Via P.A. Mattioli 4, I-53100, Siena, Italy aut Sexual Plant Reproduction Springer-Verlag 9/4(1996-07-01), 233-237 0934-0882 9:4<233 1996 9 497 https://doi.org/10.1007/BF02173104 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02173104 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tirlapur Uday Dipartimento di Biologia Ambientale, Universita di Siena, Via P.A. Mattioli 4, I-53100, Siena, Italy aut NATIONALLICENCE 700 1- Faleri Claudia Dipartimento di Biologia Ambientale, Universita di Siena, Via P.A. Mattioli 4, I-53100, Siena, Italy aut NATIONALLICENCE 700 1- Cresti Mauro Dipartimento di Biologia Ambientale, Universita di Siena, Via P.A. Mattioli 4, I-53100, Siena, Italy aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/4(1996-07-01), 233-237 0934-0882 9:4<233 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer