caa a22 4500 477053483 CHVBK 20180405111343.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02173097 doi (NATIONALLICENCE)springer-10.1007/BF02173097 Gene dispersal from transgenic crops [Elektronische Daten] II. Hybridization between oilseed rape and the wild hoary mustard [Eric Lefol, Alain Fleury, Henri Darmency] The risk of release of genetically modified oilseed rape (Brassica napus) was investigated in relation to interspecific gene flow with hoary mustard (Hirschfeldia incana). Microscopic studies showed polymorphism within the population of hoary mustard for pollen germination on oilseed rape flowers. The transgenic herbicide-resistant and a commercial cultivar of oilseed rape were not different for pollen behaviour and ovule fertilization. Pollen tube growth was slow and erratic in interspecific crosses. Fertilization efficiency of oilseed rape and hoary mustard pollen in interspecific crosses was 15% and 1.3%, respectively, of that in intraspecific crosses. This unequal efficiency in reciprocal crosses was confirmed by hybrid seed set in pods. There was no post-zygotic barrier to the development of hybrid embryos in hoary mustard pods. Up to 26 spontaneous hybrids per male sterile oilseed rape plant, and one per hoary mustard plant, were obtained in field experiments. Hybrids were identified by isozyme electrophoresis, morphology and cytology. All hybrids were triploid with 26 chromosomes, and had low fertility. They produced 0.5 seeds per plant after spontaneous backcrossing with hoary mustard. Some of these descendants were produced from unreduced gametes. Our results suggest that gene flow is likely to occur, but its actual frequency under crop growing conditions remains to be estimated. Springer-Verlag, 1996 Biosafety nationallicence Gene flow nationallicence Hoary mustard nationallicence Interspecific hybrid nationallicence Oilseed rape Pollination nationallicence Lefol Eric Laboratoire de Malherbologie, Institut National de la Recherche Agronomique, BV 1540, 21034, Dijon, France aut Fleury Alain Laboratoire de Malherbologie, Institut National de la Recherche Agronomique, BV 1540, 21034, Dijon, France aut Darmency Henri Laboratoire de Malherbologie, Institut National de la Recherche Agronomique, BV 1540, 21034, Dijon, France aut Sexual Plant Reproduction Springer-Verlag 9/4(1996-07-01), 189-196 0934-0882 9:4<189 1996 9 497 https://doi.org/10.1007/BF02173097 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02173097 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lefol Eric Laboratoire de Malherbologie, Institut National de la Recherche Agronomique, BV 1540, 21034, Dijon, France aut NATIONALLICENCE 700 1- Fleury Alain Laboratoire de Malherbologie, Institut National de la Recherche Agronomique, BV 1540, 21034, Dijon, France aut NATIONALLICENCE 700 1- Darmency Henri Laboratoire de Malherbologie, Institut National de la Recherche Agronomique, BV 1540, 21034, Dijon, France aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/4(1996-07-01), 189-196 0934-0882 9:4<189 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer