caa a22 4500 477053513 CHVBK 20180405111343.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02173100 doi (NATIONALLICENCE)springer-10.1007/BF02173100 Efficient microspore embryogenesis in wheat ( Triticum aestivum L.) induced by starvation at high temperature [Elektronische Daten] [A. Touraev, A. Indrianto, I. Wratschko, O. Vicente, E. Heberle-Bors] We have established an efficient method to induce embryo formation from isolated wheat (Triticum aestivum L.) microspores. Culture of excised anthers under starvation and heat shock conditions induced the formation of embryogenic microspores at high frequency in nine Austrian winter wheat genotypes, including cultivars that had been considered as recalcitrant in anther culture. Percoll gradient centrifugation of the mechanically isolated microspores allowed us to obtain homogeneous populations of embryogenic microspores in all genotypes which, after transfer to a rich medium containing immature ovaries for conditioning, divided and produced globular embryos. Thousands of embryos were produced in one petri dish. Many of these embryos developed into plantlets after transfer to a solid medium without ovaries. Springer-Verlag, 1996 Triticum aestivum L nationallicence Microspore embryogenesis nationallicence Starvation nationallicence Heat shock nationallicence Haploid production nationallicence Touraev A. Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9, A-1030, Vienna, Austria aut Indrianto A. Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9, A-1030, Vienna, Austria aut Wratschko I. Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9, A-1030, Vienna, Austria aut Vicente O. Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9, A-1030, Vienna, Austria aut Heberle-Bors E. Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9, A-1030, Vienna, Austria aut Sexual Plant Reproduction Springer-Verlag 9/4(1996-07-01), 209-215 0934-0882 9:4<209 1996 9 497 https://doi.org/10.1007/BF02173100 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02173100 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Touraev A. Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9, A-1030, Vienna, Austria aut NATIONALLICENCE 700 1- Indrianto A. Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9, A-1030, Vienna, Austria aut NATIONALLICENCE 700 1- Wratschko I. Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9, A-1030, Vienna, Austria aut NATIONALLICENCE 700 1- Vicente O. Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9, A-1030, Vienna, Austria aut NATIONALLICENCE 700 1- Heberle-Bors E. Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9, A-1030, Vienna, Austria aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/4(1996-07-01), 209-215 0934-0882 9:4<209 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer