caa a22 4500 47705353X CHVBK 20180405111343.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02173105 doi (NATIONALLICENCE)springer-10.1007/BF02173105 The nuclear lamina in male generative cells of Ginkgo biloba [Elektronische Daten] [J. Wang, C. Yang, Z. Zhai] Using selective extraction and diethylene glycol distearate embedment and embedment-free electron microscopy, we demonstrated nuclear lamina-like structures in sperm cells ofGinkgo biloba. A well-organized nuclear matrix network was also observed. Further studies were undertaken to determine whether or not lamin-like components exist in the pollen and sperm cells. Immunofluorescence staining using monoclonal antibodies against different animal lamins revealed lamins localized in the nuclear compartment of the sperm cells. Western blotting showed that in pollen grains there are two positive crossreaction bands at 66 kDa and 86 kDa, recognized by antibodies specific to animal lamins; in sperm cells there was only one, at 66 kDa. These results indicate that nuclear lamina containing both A-type and B-type lamins was present in male generative cells ofG. biloba. The data imply that plant lamins share some homology with animal lamins and may be conserved during evolution. Springer-Verlag, 1996 Nuclear lamina nationallicence Ginkgo biloba nationallicence Selective extraction nationallicence Western blotting nationallicence Wang J. College of Life Sciences, Peking University, 100871, Beijing, P.R. China aut Yang C. College of Life Sciences, Peking University, 100871, Beijing, P.R. China aut Zhai Z. College of Life Sciences, Peking University, 100871, Beijing, P.R. China aut Sexual Plant Reproduction Springer-Verlag 9/4(1996-07-01), 238-242 0934-0882 9:4<238 1996 9 497 https://doi.org/10.1007/BF02173105 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02173105 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang J. College of Life Sciences, Peking University, 100871, Beijing, P.R. China aut NATIONALLICENCE 700 1- Yang C. College of Life Sciences, Peking University, 100871, Beijing, P.R. China aut NATIONALLICENCE 700 1- Zhai Z. College of Life Sciences, Peking University, 100871, Beijing, P.R. China aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/4(1996-07-01), 238-242 0934-0882 9:4<238 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer