caa a22 4500 477053572 CHVBK 20180405111343.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02152700 doi (NATIONALLICENCE)springer-10.1007/BF02152700 The pollen-specific LIM protein PLIM-1 from sunflower binds nucleic acids in vitro [Elektronische Daten] [Rachel Baltz, Jean-Luc Evrard, Val/'erie Bourdon, Andr/'e Steinmetz] The protein PLIM-1 (formerly SF3) from sunflower is expressed exclusively in mature, free pollen. It contains two LIM domains associated with an acidic C-terminus comprising six copies of the pentapeptide motif (A,T,S) (E,D) TQN. We have expressed the pollen protein as well as some of its mutant forms inEscherichia coli and have used the bacterially produced proteins to study interactions with nucleic acids. Our studies show that the protein binds DNA and RNA in vitro to form large complexes, while mutant polypeptides containing either a single LIM domain or a destabilized first or second LIM domain do not. Although these data suggest that the biological function of PLIM-1 involves interactions with nucleic acids, its role in pollen development remains unclear. Springer-Verlag, 1996 Helianthus annuus L nationallicence Mature free pollen nationallicence Zinc finger nationallicence DNA binding nationallicence RNA binding nationallicence Baltz Rachel Institut de Biologie Mol/'eculaire des Plantes du CNRS, Universit/'e Louis Pasteur, 12 rue du G/'en/'eral Zimmer, F-67084, Strasbourg Cedex, France aut Evrard Jean-Luc Institut de Biologie Mol/'eculaire des Plantes du CNRS, Universit/'e Louis Pasteur, 12 rue du G/'en/'eral Zimmer, F-67084, Strasbourg Cedex, France aut Bourdon Val/'erie Institut de Biologie Mol/'eculaire des Plantes du CNRS, Universit/'e Louis Pasteur, 12 rue du G/'en/'eral Zimmer, F-67084, Strasbourg Cedex, France aut Steinmetz Andr/'e Institut de Biologie Mol/'eculaire des Plantes du CNRS, Universit/'e Louis Pasteur, 12 rue du G/'en/'eral Zimmer, F-67084, Strasbourg Cedex, France aut Sexual Plant Reproduction Springer-Verlag 9/5(1996-09-01), 264-268 0934-0882 9:5<264 1996 9 497 https://doi.org/10.1007/BF02152700 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02152700 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baltz Rachel Institut de Biologie Mol/'eculaire des Plantes du CNRS, Universit/'e Louis Pasteur, 12 rue du G/'en/'eral Zimmer, F-67084, Strasbourg Cedex, France aut NATIONALLICENCE 700 1- Evrard Jean-Luc Institut de Biologie Mol/'eculaire des Plantes du CNRS, Universit/'e Louis Pasteur, 12 rue du G/'en/'eral Zimmer, F-67084, Strasbourg Cedex, France aut NATIONALLICENCE 700 1- Bourdon Val/'erie Institut de Biologie Mol/'eculaire des Plantes du CNRS, Universit/'e Louis Pasteur, 12 rue du G/'en/'eral Zimmer, F-67084, Strasbourg Cedex, France aut NATIONALLICENCE 700 1- Steinmetz Andr/'e Institut de Biologie Mol/'eculaire des Plantes du CNRS, Universit/'e Louis Pasteur, 12 rue du G/'en/'eral Zimmer, F-67084, Strasbourg Cedex, France aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/5(1996-09-01), 264-268 0934-0882 9:5<264 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer