caa a22 4500 477053599 CHVBK 20180405111343.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02152698 doi (NATIONALLICENCE)springer-10.1007/BF02152698 Stigma development in Nicotiana tabacum. Cell death in transgenic plants as a marker to follow cell fate at high resolution [Elektronische Daten] [M. Wolters-Arts, J. Derksen, J. Kooijman, C. Mariani] Pistil development was studied in transgenic tobacco plants in which the stigma is ablated by expression of a stigma-specific cytotoxic gene. These plants offer a tool to investigate the process of differentiation of the secretory zone, in that cell death caused by barnase activity provides a marker to follow cell fate at high resolution. After fusion of the carpel walls in the region most distal from the ovary, the epidermal cells begin to divide in both wild-type and stigmaless plants. Divisions of the L1 layer of the pistil are immediately followed by the morphogenetic events that lead to three different cell types: rounded-angular cells showing an equal number of anti- and periclinal divisions, cells that are more oblong forming the transition zone, and the square cells of the transmitting tissue dividing mostly anticlinally with respect to the original carpel wall. In the stigmaless plants, cell death caused by the expression ofSTIG 1-barnase begins at stage −1 and proceeds gradually, but is always associated with round epidermal cells and with angular-rounded cells underneath them. Studies at the ultrastructural level show that cell death caused by barnase activity occurs first in solitary cells and gradually extends to groups of cells.In situ hybridizations using the STIG 1 RNA probe in wild-type pistils confirm these results. Most likely, the cells in whichSTIG 1 is expressed are those that have just differentiated into the secretory cell type. Our results indicate that the transition zone or neck is autonomously differentiated from the secretory zone and the transmitting tissue. Furthermore, our results indicate that in both wild-type and stigmaless pistils secretion of lipids most likely occurs through the plasmodesmata. This observation suggests that bulk transport can occur via plasmodesmata. Springer-Verlag, 1996 Nicotiana tabacum nationallicence Stigma nationallicence Ablation Development nationallicence Lipid secretion nationallicence Wolters-Arts M. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands aut Derksen J. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands aut Kooijman J. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands aut Mariani C. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands aut Sexual Plant Reproduction Springer-Verlag 9/5(1996-09-01), 243-254 0934-0882 9:5<243 1996 9 497 https://doi.org/10.1007/BF02152698 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02152698 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wolters-Arts M. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Derksen J. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Kooijman J. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Mariani C. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/5(1996-09-01), 243-254 0934-0882 9:5<243 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer