caa a22 4500 477053637 CHVBK 20180405111343.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02152701 doi (NATIONALLICENCE)springer-10.1007/BF02152701 Arabinogalactan proteins at the cell surface of Brassica sperm and Lilium sperm and generative cells [Elektronische Daten] [Darlene Southworth, Stephen Kwiatkowski] Antibodies to arabinogalactan proteins were tested for binding to sperm cells ofBrassica campestris and to generative cells and sperm ofLilium longiflorum. Two monoclonal antibodies, JIM8 and JIM13, bound toBrassica sperm in pollen grains and pollen tubes and to isolated sperm. Sperm pairs retained within the vegetative cell inner plasma membrane fluoresced more brightly than single sperm, indicating that the vegetative cell inner plasma membrane that surrounds sperm pairs also contains arabinogalactan proteins. Isolated sperm pairs exhibited a uniform fluorescence while single sperm had patches of fluorescence. InLilium, isolated generative cells and single sperm cells bound antibodies in a patchy pattern. Antibodies to arabinogalactan proteins may be useful in describing the overall shape of sperm cells and for identifying sperm among other cell types. Springer-Verlag, 1996 Arabinogalactan proteins nationallicence Brassica campestris nationallicence Generative cell nationallicence JIM8 nationallicence JIM13 nationallicence Lilium longiflorum nationallicence Sperm (plant) nationallicence Southworth Darlene Department of Biology, Southern Oregon State College, 97520, Ashland, OR, USA aut Kwiatkowski Stephen Department of Biology, Southern Oregon State College, 97520, Ashland, OR, USA aut Sexual Plant Reproduction Springer-Verlag 9/5(1996-09-01), 269-272 0934-0882 9:5<269 1996 9 497 https://doi.org/10.1007/BF02152701 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02152701 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Southworth Darlene Department of Biology, Southern Oregon State College, 97520, Ashland, OR, USA aut NATIONALLICENCE 700 1- Kwiatkowski Stephen Department of Biology, Southern Oregon State College, 97520, Ashland, OR, USA aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/5(1996-09-01), 269-272 0934-0882 9:5<269 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer