caa a22 4500 477053718 CHVBK 20180405111343.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02152704 doi (NATIONALLICENCE)springer-10.1007/BF02152704 Quantitative analysis of ultrastructural changes during zygotic and somatic embryogenesis in pearl millet ( Pennisetum glaucum [L.] R. Br.) [Elektronische Daten] [Mark Taylor, Indra Vasil] Ultrastructural changes during zygotic and somatic embryogenesis in pearl millet (Pennisetum glaucum [L.] R. Br.) were quantified using morphometric techniques. The total area per cell profile and the cell volume percentage of the whole cell, endoplasmic reticulum (ER), Golgi bodies, mitochondria, nuclei, lipids, plastids, starch grains and vacuoles were measured and comparisons made between three zygotic and three somatic embryo developmental stages. All measurements were taken from scutellar or scutellar-derived cells. Zygotic embryogenesis was characterized by increases in cell size, lipids, plastids, starch, Golgi bodies, mitochondria and ER. Somatic embryogenesis was characterized by two phases of cell development: (1) the dedifferentiation of scutellar cells involving a reduction in cell and vacuole size and an increase in cell activity during somatic proembryoid formation and (2) the development of somatic embryos in which most cell organelle quantities returned to values found in late coleoptile or mature predesiccation zygotic stages. In summary, although their developmental pathways differed, the scutella of somatic embryos displayed cellular variations which were within the ranges observed for later stages of zygotic embryogenesis. Springer-Verlag, 1996 Somatic embryogenesis nationallicence Ultrastructure nationallicence Pennisetum nationallicence Poaceae nationallicence Morphometrics nationallicence Taylor Mark Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Forida, 32611-0690, Gainesville, Florida, USA aut Vasil Indra Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Forida, 32611-0690, Gainesville, Florida, USA aut Sexual Plant Reproduction Springer-Verlag 9/5(1996-09-01), 286-298 0934-0882 9:5<286 1996 9 497 https://doi.org/10.1007/BF02152704 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02152704 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taylor Mark Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Forida, 32611-0690, Gainesville, Florida, USA aut NATIONALLICENCE 700 1- Vasil Indra Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Forida, 32611-0690, Gainesville, Florida, USA aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/5(1996-09-01), 286-298 0934-0882 9:5<286 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer