caa a22 4500 477053750 CHVBK 20180405111343.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02153053 doi (NATIONALLICENCE)springer-10.1007/BF02153053 PREM-2, a copia-type retroelement in maize is expressed preferentially in early microspores [Elektronische Daten] [Michael Turcich, Amana Bokhari-Riza, Douglas Hamilton, Caiping He, Walter Messier, C. Stewart, J. Mascarenhas] We have isolated, by screening a genomic library, a retroelement from maize designated PREM-2 (pollen retroelement maize-2), which is expressed in a tissue-specific manner. RNA transcripts of the PREM-2 family are found in the microspore but not in more mature pollen or in any of the vegetative tissues examined. The expression of PREM-2 elements in the uninucleate microspore provides an explanation for the genetic transmission of genomic rearrangements caused by the transposition of retroelements. PREM-2 elements are very abundant and are estimated to constitute about 5% of the maize genome and could possibly have played an important role in the determination of genome structure and in the generation of repetitive sequences in maize. The entire PREM-2 element is 9439 by long. The LTRs of PREM-2 are 1307 by in length. The internal region between the 5′ and 3′ LTRs contains 6825 by and shares homology to the gag, pro, int, RT, and RNaseH regions of copia-type retroelements. PREM-2 elements have been found in close proximity with several maize genes registered in GenBank. The presence of PREM-2 sequence in the exact 5' flanking position of three polygalacturonase genes expressed in pollen, has been used to examine the evolution of the polygalacturonase multigene family in maize and to estimate the time of the PREM-2 integration event. Springer-Verlag, 1996 Maize nationallicence Retrotransposon nationallicence Microspore expression nationallicence Polygalacturonase genes nationallicence Genome structure nationallicence Turcich Michael Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut Bokhari-Riza Amana Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut Hamilton Douglas Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut He Caiping Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut Messier Walter Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut Stewart C. Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut Mascarenhas J. Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut Sexual Plant Reproduction Springer-Verlag 9/2(1996-03-01), 65-74 0934-0882 9:2<65 1996 9 497 https://doi.org/10.1007/BF02153053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02153053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Turcich Michael Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut NATIONALLICENCE 700 1- Bokhari-Riza Amana Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut NATIONALLICENCE 700 1- Hamilton Douglas Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut NATIONALLICENCE 700 1- He Caiping Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut NATIONALLICENCE 700 1- Messier Walter Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut NATIONALLICENCE 700 1- Stewart C. Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut NATIONALLICENCE 700 1- Mascarenhas J. Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, Albany, NY, USA aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/2(1996-03-01), 65-74 0934-0882 9:2<65 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer