caa a22 4500 477053955 CHVBK 20180405111344.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02153054 doi (NATIONALLICENCE)springer-10.1007/BF02153054 A style-specific 120-kDa glycoprotein enters pollen tubes of Nicotiana alata in vivo [Elektronische Daten] [Jan Lind, Ingrid Bönig, Adrienne Clarke, Marilyn Anderson] Pistils ofNicotiana alata (Link et Otto) contain an abundant, style-specific glycoprotein (120 kDa) that is rich in hydroxyproline and has both extensin-like and arabinogalactan-protein-like carbohydrate substituents. An antibody specific for the protein backbone of the glycoprotein was used to localise the glycoprotein in both unpollinated and pollinated pistils. The glycoprotein is evenly distributed in the extracellular matrix of the style transmitting tract of unpollinated pistils and, despite the presence of extensin-like carbohydrate substituents, is not associated with the walls of the transmitting tract cells. In pollinated pistils the 120-kDa glycoprotein is concentrated in the extracellular matrix adjacent to pollen tubes, and is also present in the cytoplasm and the cell walls of pollen tubes. Pollen tubes grown in vitro do not contain the 120-kDa glycoprotein unless it is added to the growth medium, suggesting that the 120kDa glycoprotein located in pistil-grown pollen tubes is derived from the extracellular matrix of the transmitting tract. Springer-Verlag, 1996 Hydroxyproline-rich glycoprotein nationallicence Pistil nationallicence Pollen tubes nationallicence Self-incompatibility nationallicence Lind Jan Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut Bönig Ingrid Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut Clarke Adrienne Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut Anderson Marilyn Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut Sexual Plant Reproduction Springer-Verlag 9/2(1996-03-01), 75-86 0934-0882 9:2<75 1996 9 497 https://doi.org/10.1007/BF02153054 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02153054 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lind Jan Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Bönig Ingrid Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Clarke Adrienne Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Anderson Marilyn Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 773 0- Sexual Plant Reproduction Springer-Verlag 9/2(1996-03-01), 75-86 0934-0882 9:2<75 1996 9 497 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer