caa a22 4500 47705417X CHVBK 20180405111344.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02644778 doi (NATIONALLICENCE)springer-10.1007/BF02644778 Continuous monitoring of hydroperoxide-induced peroxidation in human erythrocytes by low-level chemiluminescence [Elektronische Daten] [A. Yeşilkaya, A. Yeĝin, G. Yücel, G. Alicigüzel, T. Aksu] We studied the effect of cumene hydroperoxide, t-butyl hydroperoxide, and hydrogen peroxide on intact healthy human erytyrocytes (15 g hemoglobin/dl) using chemiluminescence to monitor peroxidation. We measured the chemiluminescence spectrum, the process of hemolysis, the pH shift, and absorbance spectrum during the incubation with chemicals producing oxidative stress. Maximum chemiluminescence was reached with cumene hydroperoxide at about 50 min, but with t-butyl hydroperoxide at 100 min. The effect of organic hydroperoxide was concentration dependent, whereas the effect of hydrogen peroxide was independent of concentration. Peroxides induced hemolysis after 30 min. The pH shift to alkaline was observed in the first 20-min period. Incubation with organic hydroperoxides induced a decrease in absorption at 580, 545, and 345 nm. Hydrogen peroxide induced a decrease in the same period of time but this returned to the normal range by 120 min. There was no change in absorption at 420 nm with any of the peroxidative agents. Our results suggest that low-level chemiluminescence is a useful model for studying hydroperoxide-induced peroxidation in human erythrocytes. Springer-Verlag, 1996 Peroxidation nationallicence Low-level chemiluminescence nationallicence Hydroperoxides nationallicence Erythrocytes nationallicence Yeşilkaya A. School of Medicine, Department of Biochemistry, Akdeniz University, TR-07070, Antalya, Turkey aut Yeĝin A. School of Medicine, Department of Biochemistry, Akdeniz University, TR-07070, Antalya, Turkey aut Yücel G. School of Medicine, Department of Biochemistry, Akdeniz University, TR-07070, Antalya, Turkey aut Alicigüzel G. School of Medicine, Department of Biochemistry, Akdeniz University, TR-07070, Antalya, Turkey aut Aksu T. School of Medicine, Department of Biochemistry, Akdeniz University, TR-07070, Antalya, Turkey aut International Journal of Clinical and Laboratory Research Springer-Verlag 26/1(1996-03-01), 60-68 0940-5437 26:1<60 1996 26 599 https://doi.org/10.1007/BF02644778 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02644778 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yeşilkaya A. School of Medicine, Department of Biochemistry, Akdeniz University, TR-07070, Antalya, Turkey aut NATIONALLICENCE 700 1- Yeĝin A. School of Medicine, Department of Biochemistry, Akdeniz University, TR-07070, Antalya, Turkey aut NATIONALLICENCE 700 1- Yücel G. School of Medicine, Department of Biochemistry, Akdeniz University, TR-07070, Antalya, Turkey aut NATIONALLICENCE 700 1- Alicigüzel G. School of Medicine, Department of Biochemistry, Akdeniz University, TR-07070, Antalya, Turkey aut NATIONALLICENCE 700 1- Aksu T. School of Medicine, Department of Biochemistry, Akdeniz University, TR-07070, Antalya, Turkey aut NATIONALLICENCE 773 0- International Journal of Clinical and Laboratory Research Springer-Verlag 26/1(1996-03-01), 60-68 0940-5437 26:1<60 1996 26 599 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer