caa a22 4500 477054285 CHVBK 20180405111344.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02592981 doi (NATIONALLICENCE)springer-10.1007/BF02592981 Contrast media are incomplete secretagogues acting on human basophils and mast cells isolated from heart and lung, but not skin tissue [Elektronische Daten] [A. Genovese, C. Stellato, V. Patella, B. Lamparter-Schummert, G. de Crescenzo, M. Adt, G. Marone] To investigate the mechanisms of anaphylactoid reactions to radiocontrast media, in vitro mediator release induced by three iodinated contrast agents was examined using peripheral blood basophils and mast cells purified from human lung parenchyma, heart, and skin tissues. Three iodinated contrast agents, sodium and meglumine salts of ioxaglic acid, sodium and meglumine salts of ioxithalamic acid, and ioversol, were incubated with basophils purified from peripheral blood and human mast cells isolated and purified from different anatomical sites. Release of preformed (histamine and tryptase) and de novo synthesized mediators (prostaglandin D2 and leukotriene C4) into the supernatans was determined at various contrast medium concentrations after incubation for 60 min. Ioxaglate (0.2-0.3 M), ioxithalamate (0.3-0.5 M), and to a lesser extent ioversol (0.3-0.5 M) induced histamine release from basophils in a concentration-dependent manner. All three induced the release of preformed mediators (histamine and tryptase) from human lung, but not from skin mast cells. They also induced histamine and tryptase release from human heart mast cells. However, they did not induce the de novo synthesis of leukotriene C4 or prostaglandin D2 from human basophils or any type of mast cell examined. Cross-linking of IgE by anti-IgE induced the release of leukotriene C4 or prostaglandin D2 from human basophils or mast cells. Mannitol, an osmotic stimulus, induced the release of histamine from human basophils, but to a lesser extent from mast cells. These results show that different contrast media can differ in their ability to release mediators from enriched preparations of human basophils and mast cells. The three contrast agents examined act on basophils and mast cells as incomplete secretagogues, causing the release of preformed mediators, but not the de novo synthesis of chemical mediators. It may be useful to measure plasma tryptase levels to detect adverse reactions caused by iodinated radiographic contrast materials. Springer-Verlag, 1996 Basophils nationallicence Contrast media nationallicence Histamine nationallicence Mast cells nationallicence Tryptase nationallicence Genovese A. Division of Clinical Immunology and Allergy, University of Naples Federico II, School of Medicine, Via S. Pansini 5, I-80131, Naples, Italy aut Stellato C. Division of Clinical Immunology and Allergy, University of Naples Federico II, School of Medicine, Via S. Pansini 5, I-80131, Naples, Italy aut Patella V. Division of Clinical Immunology and Allergy, University of Naples Federico II, School of Medicine, Via S. Pansini 5, I-80131, Naples, Italy aut Lamparter-Schummert B. Department of Anesthesiology, Deutsches Herzzentrum Berlin, Berlin, Germany aut de Crescenzo G. Division of Clinical Immunology and Allergy, University of Naples Federico II, School of Medicine, Via S. Pansini 5, I-80131, Naples, Italy aut Adt M. Department of Anesthesiology, Deutsches Herzzentrum Berlin, Berlin, Germany aut Marone G. Division of Clinical Immunology and Allergy, University of Naples Federico II, School of Medicine, Via S. Pansini 5, I-80131, Naples, Italy aut International Journal of Clinical and Laboratory Research Springer-Verlag 26/3(1996-09-01), 192-198 0940-5437 26:3<192 1996 26 599 https://doi.org/10.1007/BF02592981 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02592981 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Genovese A. Division of Clinical Immunology and Allergy, University of Naples Federico II, School of Medicine, Via S. Pansini 5, I-80131, Naples, Italy aut NATIONALLICENCE 700 1- Stellato C. Division of Clinical Immunology and Allergy, University of Naples Federico II, School of Medicine, Via S. Pansini 5, I-80131, Naples, Italy aut NATIONALLICENCE 700 1- Patella V. Division of Clinical Immunology and Allergy, University of Naples Federico II, School of Medicine, Via S. Pansini 5, I-80131, Naples, Italy aut NATIONALLICENCE 700 1- Lamparter-Schummert B. Department of Anesthesiology, Deutsches Herzzentrum Berlin, Berlin, Germany aut NATIONALLICENCE 700 1- de Crescenzo G. Division of Clinical Immunology and Allergy, University of Naples Federico II, School of Medicine, Via S. Pansini 5, I-80131, Naples, Italy aut NATIONALLICENCE 700 1- Adt M. Department of Anesthesiology, Deutsches Herzzentrum Berlin, Berlin, Germany aut NATIONALLICENCE 700 1- Marone G. Division of Clinical Immunology and Allergy, University of Naples Federico II, School of Medicine, Via S. Pansini 5, I-80131, Naples, Italy aut NATIONALLICENCE 773 0- International Journal of Clinical and Laboratory Research Springer-Verlag 26/3(1996-09-01), 192-198 0940-5437 26:3<192 1996 26 599 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer