caa a22 4500 477054390 CHVBK 20180405111344.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02592983 doi (NATIONALLICENCE)springer-10.1007/BF02592983 The effects of detergents on t-butyl hydroperoxide-induced chemiluminescence [Elektronische Daten] [S. Gumüslü, A. Erkiliç, G. Yücel, M. Serteser, T. Özben] We examined the effects of Triton X-100, digitonin, sodium dodecyl sulfate, taurocholic acid, and cetylpyridinium chloride on hemoglobin-catalyzed and t-butyl hydroperoxide-induced chemiluminescence. The experimental system contained hemoglobin, luminol, t-butyl hydroperoxide, and different concentrations of detergents (5-100 mg/dl) in TRIS-HCl buffer. Control assays were performed by excluding detergents. Chemiluminescence was detected using a liquid scintillation counter in single photon mode. All concentrations chosen for each detergent reduced the maximum chemiluminescence value and retarded the time that maximum chemiluminescence occurred. The most prominent reduction in maximum chemiluminescence was observed with 50 and 100 mg/dl digitonin. The smallest reduction was observed with 5 mg/dl sodium dodecyl sulfate, without retardation of the time that maximum chemiluminescence occurred. Our aim was to use detergents in membrane-containing experimental systems and hence to identify the detergent with the least effect on chemiluminescence. Our results suggest that sodium dodecyl sulfate is the most suitable detergent for chemiluminescence studies in membrane systems. Springer-Verlag, 1996 Triton X-100 nationallicence Digitonin nationallicence Sodium dodecyl sulfate nationallicence Taurocholic acid nationallicence Chemiluminescence nationallicence Gumüslü S. Department of Biochemistry, Dumlupinar Bulvari, Akdeniz University, School of Medicine, Kampüs Alani, PK. 68, TR-07070, Antalya, Turkey aut Erkiliç A. Department of Biochemistry, Dumlupinar Bulvari, Akdeniz University, School of Medicine, Kampüs Alani, PK. 68, TR-07070, Antalya, Turkey aut Yücel G. Department of Biochemistry, Dumlupinar Bulvari, Akdeniz University, School of Medicine, Kampüs Alani, PK. 68, TR-07070, Antalya, Turkey aut Serteser M. Department of Biochemistry, Dumlupinar Bulvari, Akdeniz University, School of Medicine, Kampüs Alani, PK. 68, TR-07070, Antalya, Turkey aut Özben T. Department of Biochemistry, Dumlupinar Bulvari, Akdeniz University, School of Medicine, Kampüs Alani, PK. 68, TR-07070, Antalya, Turkey aut International Journal of Clinical and Laboratory Research Springer-Verlag 26/3(1996-09-01), 203-206 0940-5437 26:3<203 1996 26 599 https://doi.org/10.1007/BF02592983 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02592983 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gumüslü S. Department of Biochemistry, Dumlupinar Bulvari, Akdeniz University, School of Medicine, Kampüs Alani, PK. 68, TR-07070, Antalya, Turkey aut NATIONALLICENCE 700 1- Erkiliç A. Department of Biochemistry, Dumlupinar Bulvari, Akdeniz University, School of Medicine, Kampüs Alani, PK. 68, TR-07070, Antalya, Turkey aut NATIONALLICENCE 700 1- Yücel G. Department of Biochemistry, Dumlupinar Bulvari, Akdeniz University, School of Medicine, Kampüs Alani, PK. 68, TR-07070, Antalya, Turkey aut NATIONALLICENCE 700 1- Serteser M. Department of Biochemistry, Dumlupinar Bulvari, Akdeniz University, School of Medicine, Kampüs Alani, PK. 68, TR-07070, Antalya, Turkey aut NATIONALLICENCE 700 1- Özben T. Department of Biochemistry, Dumlupinar Bulvari, Akdeniz University, School of Medicine, Kampüs Alani, PK. 68, TR-07070, Antalya, Turkey aut NATIONALLICENCE 773 0- International Journal of Clinical and Laboratory Research Springer-Verlag 26/3(1996-09-01), 203-206 0940-5437 26:3<203 1996 26 599 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer