caa a22 4500 477054455 CHVBK 20180405111344.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02592980 doi (NATIONALLICENCE)springer-10.1007/BF02592980 Effects of nordihydroguaiaretic acid on murine antibody-dependent cellular cytotoxicity [Elektronische Daten] [J. Hernández-Gody, D. Planelles, B. Balsalobre, A. González-Molina] The purpose of this study was to analyze the effects of nordihydroguaiaretic acid, an inhibitor of the lipoxygenase pathway of arachidonic acid, on antibody-dependent cellular cytotoxicity. Antibody-dependent cellular cytotoxicity mediated by murine spleen cells was significantly inhibited by concentrations of nordihydroguaiaretic acid from 10−5 to 10−4 M (IC50=2×10−5 M). The inhibitory effect of nordihydroguaiaretic acid was also observed on antibody-dependent cellular cytotoxicity mediated by macrophage-depleted spleen cells as well as isolated macrophages. Nordihydroguaiaretic acid was highly effective when added at the beginning of the assay and was always present throughout the assay, but failed to inhibit the binding of effector and target cells. The inhibition produced by nordihydroguaiaretic acid could not be reversed by leukotriene B4, a 5-lipoxygenase product. These results suggest that arachidonic acid metabolies other than leukotriene B4 are released by different populations of spleen cells to positively regulate important events in the postbinding phase of murine antibody-dependent cellular cytotoxicity. Springer-Verlag, 1996 Antibody-dependent cellular cytotoxicity nationallicence Arachidonic acid nationallicence Lipoxygenase nationallicence Nordihydroguaiaretic acid nationallicence Mice nationallicence Hernández-Gody J. Unidad de Inmunología Experimental, Centro de Investigación, Hospital La Fe, E-46009, Valencia, Spain aut Planelles D. Unidad de Inmunología Experimental, Centro de Investigación, Hospital La Fe, E-46009, Valencia, Spain aut Balsalobre B. Departmento de Microbiología, Universidad de Valencia, Valencia, Spain aut González-Molina A. Unidad de Inmunología Experimental, Centro de Investigación, Hospital La Fe, E-46009, Valencia, Spain aut International Journal of Clinical and Laboratory Research Springer-Verlag 26/3(1996-09-01), 185-191 0940-5437 26:3<185 1996 26 599 https://doi.org/10.1007/BF02592980 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02592980 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hernández-Gody J. Unidad de Inmunología Experimental, Centro de Investigación, Hospital La Fe, E-46009, Valencia, Spain aut NATIONALLICENCE 700 1- Planelles D. Unidad de Inmunología Experimental, Centro de Investigación, Hospital La Fe, E-46009, Valencia, Spain aut NATIONALLICENCE 700 1- Balsalobre B. Departmento de Microbiología, Universidad de Valencia, Valencia, Spain aut NATIONALLICENCE 700 1- González-Molina A. Unidad de Inmunología Experimental, Centro de Investigación, Hospital La Fe, E-46009, Valencia, Spain aut NATIONALLICENCE 773 0- International Journal of Clinical and Laboratory Research Springer-Verlag 26/3(1996-09-01), 185-191 0940-5437 26:3<185 1996 26 599 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer