caa a22 4500 477054471 CHVBK 20180405111344.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF02602959 doi (NATIONALLICENCE)springer-10.1007/BF02602959 Functional characteristics of cord blood T lymphocytes after lectin and anti-CD3 stimulation [Elektronische Daten] Differences in the way T cells express activation molecules and proliferate [G. Lucivero, L. Dalla Mora, E. Bresciano, M. Loria, L. Pezone, D. Mancino] Newborns are more susceptible than adults to infections, which suggests a relative immaturity of the immune system early after birth. Cord blood T cells differ significantly both in surface phenotype and function from adult T cells. We examined the proliferation and expression of activation molecules by lymphocytes isolated from umbilical cord blood or peripheral blood of adults. The lymphocytes were cultured for 5 days in the presence of phytohemagglutinin, concanavalin A, or anti-CD3 monoclonal antibody. Cord blood T cells expressed the CD45RA molecule, while a low proportion expressed the RO isoform, a marker of primed or activated lymphocytes. Furthermore, more than 95% of neonatal lymphocytes bear the CD38 molecule, but do not express the CD57 molecule. After stimulation by phytohemagglutinin or concanavalin A, the lymphocytes from newborns were activated and proliferated as efficiently as adult T cells. Anti-CD3 did not cause neonatal lymphocytes to proliferate, but these cells expressed activation molecules, such as HLA-DR antigens and the receptor for interleukin-2 and transferrin. The relevance of these findings to tolerance induction in immature cord blood T cells is discussed. Springer-Verlag, 1996 Cord blood lymphocytes nationallicence Proliferation nationallicence Activation molecules nationallicence Lucivero G. Department of Geriatrics, Gerontology and Metabolic Diseases (Allergology and Immunology Service), The 2nd University of Naples, I-80138, Naples, Italy aut Dalla Mora L. Institute of General Pathology (Immunopathology Service), The 2nd University of Naples, I-80138, Naples, Italy aut Bresciano E. Institute of General Pathology (Immunopathology Service), The 2nd University of Naples, I-80138, Naples, Italy aut Loria M. Institute of Clinical Medicine, Allergology and Immunology Section, University of Bari, I-70124, Bari, Italy aut Pezone L. Institute of General Pathology (Immunopathology Service), The 2nd University of Naples, I-80138, Naples, Italy aut Mancino D. Institute of General Pathology (Immunopathology Service), The 2nd University of Naples, I-80138, Naples, Italy aut International Journal of Clinical and Laboratory Research Springer-Verlag 26/4(1996-12-01), 255-261 0940-5437 26:4<255 1996 26 599 https://doi.org/10.1007/BF02602959 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02602959 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lucivero G. Department of Geriatrics, Gerontology and Metabolic Diseases (Allergology and Immunology Service), The 2nd University of Naples, I-80138, Naples, Italy aut NATIONALLICENCE 700 1- Dalla Mora L. Institute of General Pathology (Immunopathology Service), The 2nd University of Naples, I-80138, Naples, Italy aut NATIONALLICENCE 700 1- Bresciano E. Institute of General Pathology (Immunopathology Service), The 2nd University of Naples, I-80138, Naples, Italy aut NATIONALLICENCE 700 1- Loria M. Institute of Clinical Medicine, Allergology and Immunology Section, University of Bari, I-70124, Bari, Italy aut NATIONALLICENCE 700 1- Pezone L. Institute of General Pathology (Immunopathology Service), The 2nd University of Naples, I-80138, Naples, Italy aut NATIONALLICENCE 700 1- Mancino D. Institute of General Pathology (Immunopathology Service), The 2nd University of Naples, I-80138, Naples, Italy aut NATIONALLICENCE 773 0- International Journal of Clinical and Laboratory Research Springer-Verlag 26/4(1996-12-01), 255-261 0940-5437 26:4<255 1996 26 599 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer