caa a22 4500 477054536 CHVBK 20180405111344.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF02602956 doi (NATIONALLICENCE)springer-10.1007/BF02602956 Cytokines may influence tumor growth and spread [Elektronische Daten] An in vitro study in two human cancer cell lines [M. Panozzo, D. Basso, M. De Paoli, P. Carraro, D. Burighel, M. Plebani] Tumor spread may be favored by a reduced production and/or an enhanced degradation of extracellular matrix components (collagen, fibronectin, laminin). Most tumor cell behavior, from growth to spread, may be regulated by cytokines, the exact roles of which, however, are not yet fully understood. We here evaluate the effects of some cytokines (epidermal growth factor, transforming growth factor-β1, interleukin-1α, and interleukin-1β) on both cell growth and the production of the aminoterminal peptide of type III procollagen, the urokinase plasminogen activator, and the plasminogen activator inhibitor-1 in neoplastic cell lines originating in the pancreas and colon. Cells were stimulated daily with the above cytokines and the aminoterminal peptide of type III procollagen, urokinase plasminogen activator, and plasminogen activator inhibitor-1 were measured in the conditioned media. Epidermal growth factor stimulated cell growth of both cell lines. Transforming growth factor-β1 counteracted cell proliferation and stimulated type III procollagen and plasminogen activator inhibitor-1 production only in the colon cancer cell line. Interleukin-1α slightly stimulated cell growth, but inhibited plasminogen activator inhibitor-1 production in both cell lines; interleukin-1 β did not affect cell growth, but stimulated plasminogen activator inhibitor-1 production by the colon cancer cell line. Our findings suggest that transforming growth factor-β1 and interleukin-1β may have an antidiffusive effect. These results confirm that cytokine-producing cells have a potential role in stimulating or counteracting tumor growth and spread and also confirm the pivotal role of host-tumor interactions in determining the outcome of a particular neoplasia. Springer-Verlag, 1996 Cytokines nationallicence Pancreatic cancer nationallicence Colon cancer nationallicence Metastasis nationallicence Cell proliferation nationallicence Panozzo M. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut Basso D. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut De Paoli M. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut Carraro P. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut Burighel D. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut Plebani M. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut International Journal of Clinical and Laboratory Research Springer-Verlag 26/4(1996-12-01), 240-244 0940-5437 26:4<240 1996 26 599 https://doi.org/10.1007/BF02602956 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02602956 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Panozzo M. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut NATIONALLICENCE 700 1- Basso D. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut NATIONALLICENCE 700 1- De Paoli M. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut NATIONALLICENCE 700 1- Carraro P. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut NATIONALLICENCE 700 1- Burighel D. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut NATIONALLICENCE 700 1- Plebani M. Department of Laboratory Medicine, University of Padua, Via Giustiniani 2, I-35128, Padua, Italy aut NATIONALLICENCE 773 0- International Journal of Clinical and Laboratory Research Springer-Verlag 26/4(1996-12-01), 240-244 0940-5437 26:4<240 1996 26 599 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer