caa a22 4500 47705465X CHVBK 20180405111345.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02592352 doi (NATIONALLICENCE)springer-10.1007/BF02592352 Effect of substance P and its precursor α-protachykinin on intracellular free calcium concentration in human polymorphonuclear leukocytes [Elektronische Daten] [D. Nowak, E. Hrabec, J. Greger, G. Piasecka, M. Krol, P. Bialasiewicz, A. Antczak, G. Plucienniczak, A. Plucienniczak] The increase in intracellular free calcium concentration is an important step in signal transduction leading to phagocyte activation. The undecapeptide substance P can influence various functions of human polymorphonuclear leukocytes, including chemotaxis, phagocytosis, and respiratory burst. In this study we investigated the ability of low-concentration (that can occur in vivo) substance P (10−7 M) and its precursor α-protachykinin (3×10−7 M) to increase the intracellular free calcium concentration in human polymorphonuclear leukocytes. Cells isolated from ten healthy donors were incubated with substance P or α-protachykinin in 1 mM calcium medium for 5 min and the intracellular free calcium concentration was monitored using the fluorescent calcium indicator Fura-2am. Polymorphonuclear leukocytes from 40% of donors responded to both agonists. The substance P- and α-protachykinin-induced increase in intracellular free calcium concentration was 59±13 nM and 58±12 nM and the extracellular calcium influx contributed to 87±8% and 54±8% of the calcium response, respectively. α-Protachykinin released almost all the calcium from intracellular stores, while substance P mobilized only 24±5% of this calcium pool. Finally, cells that responded to a single challenge with substance P and α-protachykinin were able to increase their intracellular free calcium concentration in response to each of three consecutive stimulations with these agonists. This may be an additional mechanism by which substance P and its precursor modify the function of human polymorphonuclear leukocytes. Springer-Verlag, 1996 Substance P nationallicence α-Protachykinin nationallicence Polymorphonuclear leukocytes nationallicence Intracellular calcium nationallicence Intracellular calcium stores nationallicence Nowak D. Department of Pneumology and Allergology, Medical University of Lodz, Kopcinskiego St 22, 90-153, Lodz, Poland aut Hrabec E. Department of Biochemistry, Medical University of Lodz, Lindleya St 6, 90-131, Lodz, Poland aut Greger J. Department of Biochemistry, Medical University of Lodz, Lindleya St 6, 90-131, Lodz, Poland aut Piasecka G. Department of Pneumology and Allergology, Medical University of Lodz, Kopcinskiego St 22, 90-153, Lodz, Poland aut Krol M. Department of Pneumology and Allergology, Medical University of Lodz, Kopcinskiego St 22, 90-153, Lodz, Poland aut Bialasiewicz P. Department of Pneumology and Allergology, Medical University of Lodz, Kopcinskiego St 22, 90-153, Lodz, Poland aut Antczak A. Department of Pneumology and Allergology, Medical University of Lodz, Kopcinskiego St 22, 90-153, Lodz, Poland aut Plucienniczak G. Center of Microbiology and Virology, Polish Academy of Sciences, Dabrowskiego St 251, 93-231, Lodz, Poland aut Plucienniczak A. Laboratory of Genetic Engineering, P.O.W. 57, 98-200, Sieradz, Poland aut International Journal of Clinical and Laboratory Research Springer-Verlag 26/2(1996-07-01), 106-111 0940-5437 26:2<106 1996 26 599 https://doi.org/10.1007/BF02592352 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02592352 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nowak D. Department of Pneumology and Allergology, Medical University of Lodz, Kopcinskiego St 22, 90-153, Lodz, Poland aut NATIONALLICENCE 700 1- Hrabec E. Department of Biochemistry, Medical University of Lodz, Lindleya St 6, 90-131, Lodz, Poland aut NATIONALLICENCE 700 1- Greger J. Department of Biochemistry, Medical University of Lodz, Lindleya St 6, 90-131, Lodz, Poland aut NATIONALLICENCE 700 1- Piasecka G. Department of Pneumology and Allergology, Medical University of Lodz, Kopcinskiego St 22, 90-153, Lodz, Poland aut NATIONALLICENCE 700 1- Krol M. Department of Pneumology and Allergology, Medical University of Lodz, Kopcinskiego St 22, 90-153, Lodz, Poland aut NATIONALLICENCE 700 1- Bialasiewicz P. Department of Pneumology and Allergology, Medical University of Lodz, Kopcinskiego St 22, 90-153, Lodz, Poland aut NATIONALLICENCE 700 1- Antczak A. Department of Pneumology and Allergology, Medical University of Lodz, Kopcinskiego St 22, 90-153, Lodz, Poland aut NATIONALLICENCE 700 1- Plucienniczak G. Center of Microbiology and Virology, Polish Academy of Sciences, Dabrowskiego St 251, 93-231, Lodz, Poland aut NATIONALLICENCE 700 1- Plucienniczak A. Laboratory of Genetic Engineering, P.O.W. 57, 98-200, Sieradz, Poland aut NATIONALLICENCE 773 0- International Journal of Clinical and Laboratory Research Springer-Verlag 26/2(1996-07-01), 106-111 0940-5437 26:2<106 1996 26 599 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer