caa a22 4500 477057489 CHVBK 20180405111350.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/s002280050081 doi (NATIONALLICENCE)springer-10.1007/s002280050081 Coumarin 7-hydroxylation in long-term adherents of a strict uncooked vegan diet [Elektronische Daten] [A.-L. Rauma, A. Rautio, M. Pasanen, O. Pelkonen, R. Törrönen, H. Mykkänen] Objective: Coumarin 7-hydroxylation was investigated in 21 Finnish vegans (20 females, one male) consuming a strict, uncooked vegan diet ("living food diet”) and in their matched omnivorous controls, by means of an in vivo coumarin test. Method: A capsule containing 5 mg of coumarin (Venalot®) was taken after an overnight fast, and urine samples were collected before and 2, 4 and 6 h after the drug administration. The extent and rate of urinary excretion of 7-hydroxycoumarin was determined using HPLC. Results: The total urinary excretion of 7-hydroxycoumarin during 6 h was 58 (range 23-85) and 64 (range 39-92)% of the administred dose in the vegan and control groups. The coumarin index (excretion of 7-hydroxycoumarin during the first 2 h as percentage of total excretion) was 72% in the vegan and 78% in the control groups. A negative correlation was observed between the coumarin index and the consumption of wheatgrass juice by the vegans (r = −0.60, P < 0.01, n = 21). Proportion of slow hydroxylators (excreting 7-hydroxycoumarin after 4 h) was not statistically different between the groups (5/21 in the vegans vs 8/20 in the controls). Conclusion: According to the present study, the clearly different dietary patterns and nutrient intakes between the vegans and the omnivores resulted in similar extent and rate of 7-hydroxycoumarin formation, indicating only a minor effect on coumarin hydroxylase (CYP2A6) activity by the plant substances in the uncooked vegan diet. Springer-Verlag Berlin Heidelberg, 1996 Key words Coumarin nationallicence Coumarin 7-hydroxylation nationallicence Vegan diet nationallicence Biotransformation nationallicence Rauma A.-L Department of Clinical Nutrition, University of Kuopio, PO Box 1627, SF-70211 Kuopio, Finland, FI aut Rautio A. Department of Pharmacology and Toxicology, University of Oulu, SF-90220 Oulu, Finland, FI aut Pasanen M. Department of Pharmacology and Toxicology, University of Oulu, SF-90220 Oulu, Finland, FI aut Pelkonen O. Department of Pharmacology and Toxicology, University of Oulu, SF-90220 Oulu, Finland, FI aut Törrönen R. Department of Physiology, University of Kuopio, P.O. Box 1627, SF-70211 Kuopio, Finland, FI aut Mykkänen H. Department of Clinical Nutrition, University of Kuopio, PO Box 1627, SF-70211 Kuopio, Finland, FI aut https://doi.org/10.1007/s002280050081 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002280050081 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rauma A.-L Department of Clinical Nutrition, University of Kuopio, PO Box 1627, SF-70211 Kuopio, Finland, FI aut NATIONALLICENCE 700 1- Rautio A. Department of Pharmacology and Toxicology, University of Oulu, SF-90220 Oulu, Finland, FI aut NATIONALLICENCE 700 1- Pasanen M. Department of Pharmacology and Toxicology, University of Oulu, SF-90220 Oulu, Finland, FI aut NATIONALLICENCE 700 1- Pelkonen O. Department of Pharmacology and Toxicology, University of Oulu, SF-90220 Oulu, Finland, FI aut NATIONALLICENCE 700 1- Törrönen R. Department of Physiology, University of Kuopio, P.O. Box 1627, SF-70211 Kuopio, Finland, FI aut NATIONALLICENCE 700 1- Mykkänen H. Department of Clinical Nutrition, University of Kuopio, PO Box 1627, SF-70211 Kuopio, Finland, FI aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer