caa a22 4500 477058329 CHVBK 20180405111353.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00203779 doi (NATIONALLICENCE)springer-10.1007/BF00203779 Microinjection technique for pharmacological evaluation of microvascular permeability in human skin [Elektronische Daten] [I. Herrig, U. Hoffmann, M. Fischer, U. Franzeck, A. Bollinger] A new technique which combines skin microinjection of minute amounts of drugs (0.5 μl) and measurement of capillary permeability by intravital fluorescence videomicroscopy and densitometry is introduced. Glass micropipettes with a tip diameter of 7-9 μm are inserted by a micromanipulator into the stratum papillare containing the capillary loops and used for microinjection by microsyringe and special dispenser. Transcapillary diffusion of sodium fluorescein applied by intravenous bolus injection is visualised by fluorescence videomicroscopy and stored on videotape. Perivascular fluorescent light intensity (FLI) is measured in arbitrary units (AU) by videodensitometry around the site of microinjection during playback of the videotapes. The method was tested by microinjection of 0.5 μl histamine (1‰ solution) at the distal tibial plateau. Mean FLI values representing microvascular permeability were 2186 AU 10 min after microinjection of histamine, 420 AU after physiologic saline and 1228 AU after histamine combined with oral intake of 20 mg cetirizine. Cetirizine significantly reduced (P<0.01) the increased permeability induced by histamine. However, the mean values after injection of the H1-blocker were still significantly enhanced (P<0.01) when compared to the mean values observed after injection of physiologic saline solution, which provoked only minor microtrauma. The technique offers new scope for pharmacological testing in man. Springer-Verlag, 1996 Fluorescence videomicroscopy nationallicence Histamine nationallicence Cetirizine nationallicence sodium fluorescein nationallicence Herrig I. Department of Internal Medicine, Angiology Division, University Hospital, CH-8091, Zürich, Switzerland aut Hoffmann U. Department of Internal Medicine, Angiology Division, University Hospital, CH-8091, Zürich, Switzerland aut Fischer M. Department of Internal Medicine, Angiology Division, University Hospital, CH-8091, Zürich, Switzerland aut Franzeck U. Department of Internal Medicine, Angiology Division, University Hospital, CH-8091, Zürich, Switzerland aut Bollinger A. Department of Internal Medicine, Angiology Division, University Hospital, CH-8091, Zürich, Switzerland aut European Journal of Clinical Pharmacology Springer-Verlag 49/5(1996-02-01), 365-369 0031-6970 49:5<365 1996 49 228 https://doi.org/10.1007/BF00203779 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00203779 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Herrig I. Department of Internal Medicine, Angiology Division, University Hospital, CH-8091, Zürich, Switzerland aut NATIONALLICENCE 700 1- Hoffmann U. Department of Internal Medicine, Angiology Division, University Hospital, CH-8091, Zürich, Switzerland aut NATIONALLICENCE 700 1- Fischer M. Department of Internal Medicine, Angiology Division, University Hospital, CH-8091, Zürich, Switzerland aut NATIONALLICENCE 700 1- Franzeck U. Department of Internal Medicine, Angiology Division, University Hospital, CH-8091, Zürich, Switzerland aut NATIONALLICENCE 700 1- Bollinger A. Department of Internal Medicine, Angiology Division, University Hospital, CH-8091, Zürich, Switzerland aut NATIONALLICENCE 773 0- European Journal of Clinical Pharmacology Springer-Verlag 49/5(1996-02-01), 365-369 0031-6970 49:5<365 1996 49 228 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer