caa a22 4500 477061761 CHVBK 20180405111402.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00226410 doi (NATIONALLICENCE)springer-10.1007/BF00226410 Affinity of fibrinogen binding to platelet membrane glycoprotein IIb/IIIa increases with RGDS and γ chain fibrinogen peptide hybrid [Elektronische Daten] [Hiroshi Mohri, Takao Okubo] Arg-Gly-Asp (RGD)-containing peptides and the peptide unique to fibrinogen in the C-terminal domain of the γ chain are important for fibrinogen binding to platelet membrane glycoprotein (GP) II b/III a. We synthesized a unique hybrid peptide of YRGDSPLGGAKQAGDV encompassing the RGD sequence (RGDS) with the γ chain peptide (LGGAK-QAGDV). Maximum binding to thrombin-stimulated platelets was achieved within 40 minutes with the YRGDS peptide and within 20 minutes with the hybrid peptide and native fibrinogen. The platelet binding sites were 58,600 molecules with the hybrid peptide and 52,400 molecules with YRGDS. These peptides inhibited fibrinogen binding to thrombin-stimulated platelets in a dose-dependent manner. The order of inhibitory potency of these peptides was as follows: fibrinogen $$ \risingdotseq$$ hybrid peptide > YRGDS > GQQHHLGGAKQAGDV (G15). The RGES peptide had no inhibitory activity. These three peptides inhibited binding of the anti-GP II b/III a monoclonal antibody (LJ-CP8) to platelets. The hybrid peptide showed the most potent inhibitory activity, with an IC50 of 48 μM. These results suggest that the linear combined RGDS and γ chain peptide created with proline increases the affinity of binding to activated platelets. Kluwer Academic Publishers, 1996 fibrinogen nationallicence GP II b/III a nationallicence affinity nationallicence synthetic peptides nationallicence Mohri Hiroshi First Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama, Japan aut Okubo Takao First Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama, Japan aut Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/1(1996-03-01), 45-49 0929-5305 3:1<45 1996 3 11239 https://doi.org/10.1007/BF00226410 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226410 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mohri Hiroshi First Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama, Japan aut NATIONALLICENCE 700 1- Okubo Takao First Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama, Japan aut NATIONALLICENCE 773 0- Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/1(1996-03-01), 45-49 0929-5305 3:1<45 1996 3 11239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer