caa a22 4500 477061818 CHVBK 20180405111403.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00181660 doi (NATIONALLICENCE)springer-10.1007/BF00181660 Stuttering reperfusion of ischemic myocardium does not exacerbate myocardial infarction: Evidence against lethal cell reperfusion injury in the rabbit [Elektronische Daten] [Kevin Alker, Stephen Bellows, Robert Kloner] In the clinical setting of acute myocardial infarction, coronary reperfusion may occur intermittently. Whether this intermittent or stuttering reperfusion exacerbates reperfusion cell injury is not known. To investigate whether stuttering reperfusion affects the development of necrosis, anesthetized male rabbits were randomized to either control or stuttering-reperfusion groups. The control rabbits underwent 40 minutes of continuous left anterior descending (LAD) occlusion followed by 3 hours of continuous reperfusion. The stuttering-reperfusion rabbits were occluded for the same 40 minutes but were reperfused for 1 minute at 37, 39, and 41 minutes into the occlusion period. Each of these brief reperfusion periods was followed by 1 minute of occlusion. After 40 minutes of occlusion (43 minutes after initial occlusion), the stuttering-reperfusion group was continuously reperfused for 3 hours. Regional myocardial blood flow was measured during occlusion and 30 minutes after final reperfusion in both groups. Area at risk (AR, %LV) was delineated by in vivo blue dye injection and the area of necrosis (AN) by in vitro tetrazolium staining. Results: There was no difference in AR blood flow between groups, Ischemic blood flow (ml/min/g) was 0.05 ± 0.02 in the control group and 0.09 ± 0.04 in stuttering-reperfusion group. After reperfusion, blood flow in the ischemic area was 1.36 ± 0.13 in control and 1.87 ± 0.40 in stuttering-reperfusion rabbits. The AN expressed as a percentage of the AR was 43 ± 8 in the control rabbits and 36 ± 6 in the stuttering-reperfusion rabbits. Stuttering-reperfusion did not augment the development of necrosis compared with the control group. Conclusion: Reperfusing the myocardium in a stuttering fashion had no effect on the amount of necrosis and did not contribute to lethal cell reperfusion injury in this model. Kluwer Academic Publishers, 1996 stuttering reperfusion nationallicence reperfusion injury nationallicence infarct size extension nationallicence myocardial infarct size nationallicence Alker Kevin The Heart Institute, Good Samaratin Hospital and The University of Southern California, Los Angeles, California aut Bellows Stephen The Heart Institute, Good Samaratin Hospital and The University of Southern California, Los Angeles, California aut Kloner Robert The Heart Institute, Good Samaratin Hospital and The University of Southern California, Los Angeles, California aut Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/3(1996-09-01), 185-188 0929-5305 3:3<185 1996 3 11239 https://doi.org/10.1007/BF00181660 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00181660 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alker Kevin The Heart Institute, Good Samaratin Hospital and The University of Southern California, Los Angeles, California aut NATIONALLICENCE 700 1- Bellows Stephen The Heart Institute, Good Samaratin Hospital and The University of Southern California, Los Angeles, California aut NATIONALLICENCE 700 1- Kloner Robert The Heart Institute, Good Samaratin Hospital and The University of Southern California, Los Angeles, California aut NATIONALLICENCE 773 0- Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/3(1996-09-01), 185-188 0929-5305 3:3<185 1996 3 11239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer