caa a22 4500 477062059 CHVBK 20180405111403.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00133076 doi (NATIONALLICENCE)springer-10.1007/BF00133076 Dependence of human vascular cell surface proteolysis on expression of the urokinase receptor [Elektronische Daten] [Hirofumi Sawa, Craig Lundgren, Steven Brown, Satoshi Fujii] To delineate the role of binding of urokinase type plasminogen activator (uPA) to its receptor (uPAR) in the local generation of plasmin by endothelium, we transfected spontaneously transformed immortalized human vascular endothelial cells that express high levels of uPA but low levels of uPAR with human uPAR complementary DNA. Compared with nontransfected cell, the stably transformed clonal cell line exhibited (a) a > 10-fold increase in steady-state uPAR mRNA levels documented with Northern blot analysis (n = 3), (b) a 2.8-fold increase in cell surface expression of uPAR protein quantified by enzyme linked immunosorbent assay (n = 3), (c) a 2.9-fold increase in specific binding of radiolabeled single chain uPA (n = 4), and (d) markedly increased matrix adhesion. The participation of uPAR in cell surface proteolysis was apparent based on a 3.0-fold increase in cell associated plasmin activity (n = 3) and a 2.3-fold increase in lysis of noncrosslinked fibrin clots (n = 5). Thus, local generation of plasmin and consequent degradation of fibrin are likely to be promoted by cell surface localization of uPA by uPAR in cellular constituents of the vessel wall. Furthermore, genetic engineering of endothelium to enhance expression of uPAR may confer resistance to thrombosis or restenosis associated with endovascular stents. Kluwer Academic Publishers, 1996 endothelial cell nationallicence urokinase nationallicence urokinase receptor nationallicence fibrinolysis nationallicence Sawa Hirofumi Cardiovascular Division, Washington University School of Medicine, St. Louis, Missouri, USA aut Lundgren Craig Cardiovascular Division, Washington University School of Medicine, St. Louis, Missouri, USA aut Brown Steven Cardiovascular Division, Washington University School of Medicine, St. Louis, Missouri, USA aut Fujii Satoshi Cardiovascular Division, University of Vermont College of Mediane, Burlington, Vermont, USA aut Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/4(1996-12-01), 331-336 0929-5305 3:4<331 1996 3 11239 https://doi.org/10.1007/BF00133076 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00133076 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sawa Hirofumi Cardiovascular Division, Washington University School of Medicine, St. Louis, Missouri, USA aut NATIONALLICENCE 700 1- Lundgren Craig Cardiovascular Division, Washington University School of Medicine, St. Louis, Missouri, USA aut NATIONALLICENCE 700 1- Brown Steven Cardiovascular Division, Washington University School of Medicine, St. Louis, Missouri, USA aut NATIONALLICENCE 700 1- Fujii Satoshi Cardiovascular Division, University of Vermont College of Mediane, Burlington, Vermont, USA aut NATIONALLICENCE 773 0- Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/4(1996-12-01), 331-336 0929-5305 3:4<331 1996 3 11239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer