caa a22 4500 477062067 CHVBK 20180405111403.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00133078 doi (NATIONALLICENCE)springer-10.1007/BF00133078 Reduction of platelet thrombi and emboli by L-arginine during cardiopulmonary bypass in a pig model [Elektronische Daten] [Mrinal Dewanjee, Shu Wu, Mansoor Kapadvanjwala, Dibyendu De, Sumit Dewanjee, Stana Novak, Li-Chien Hsu, Richard Perryman, Aldo Serafini, George Sfakianakis, Robert Duncan, W. Dietrich, Allan Horton] We wanted to test the hypothesis that NO generation by L-arginine (LA) infusion will be beneficial in increasing blood flow to all organs to counteract the process of global ischemia during cardiopulmonary bypass (CPB) and to reduce platelet emboli by platelet inhibition. The effect of LA infusion on NO formation, vasodilation, and reduction of thromboembolic burden in organs and tissues after CPB was quantified with In-111-labeled autologous platelets in two major groups: 180 minutes CPB (CPB) and 90 minutes CPB plus 90 minutes reperfusion (RP). Platelets labeled with In-111 tropolone (650-780 μCi) were administered 24 hours before CPB and LA infusion (bolus, 10 mg/kg and infusion at 2 mg/kg/min, 21 pigs for 180 minutes CPB) in 8 groups of 30 Yorkshire pigs (30-35 kg, 6 pigs; LA 2 mg/kg/min, 3 pigs; sham-thoracotomy control, 6 pigs; unoperated control, 6 pigs). Two groups of 9 pigs (control CPB, 6 pigs; LA 2 mg/ kg/min, 3 pigs) underwent 90 minutes of CPB and 90 minutes of reperfusion. All pigs were heparinized (ACT >400 seconds); CPB was instituted with a roller pump, an oxygenator (OX: Bentley Univox, 1.8 m2), and an arterial filter (AF: 0.25 m2, Bentley) at a blood flow of 2.5-3.5 1/min. Radioactive thrombi in OX and AF and emboli in viscera, brain, and connective tissues were imaged with a gamma camera and were finally measured with an ion chamber and a gamma counter. The percent of injected platelets (mean ± SD) in the organs and tissues of all pigs was calculated. Cerebral emboli were mapped in 25 regions of both hemispheres of pig brain. Flow cytometry with antibodies to CD61 (GPIIIa) and CD62P (GMP-140: control) of porcine platelets was carried out with blood samples taken before, during, and after CPB. Coronary bypass with LA infusion decreased the amount of adherent thrombi in OX and AF (p < 0.07). The embolic burden in brain and lung also decreased. Regional cerebral mapping of In-111 platelets showed reduced emboli in almost all regions, including the medulla, hippocampus, and posterior cerebral cortex in both LA-treated groups. Flow cytometry of blood samples demonstrated the shift of equilibria from single platelet to platelet-aggregate-microparticle during CPB and steady-state level after the first 5-10 minutes of initiation of CPB. The L-arginine infusion reduced thrombi and emboli during CPB in the pig model. Kluwer Academic Publishers, 1996 cardiopulmonary bypass nationallicence Yorkshire pigs nationallicence indium-111 label nationallicence platelets nationallicence thrombi nationallicence emboli nationallicence fow-cytometry nationallicence trapped platelet-emboli nationallicence NOS gene nationallicence L-arginine nationallicence Dewanjee Mrinal Department of Radiology, University of Miami, School of Medicine, Miami, Florida, USA aut Wu Shu Department of Radiology, University of Miami, School of Medicine, Miami, Florida, USA aut Kapadvanjwala Mansoor Department of Biomedical Engineering, University of Miami, School of Medicine, Miami, Florida, USA aut De Dibyendu Department of Biomedical Engineering, University of Miami, School of Medicine, Miami, Florida, USA aut Dewanjee Sumit Department of Radiology, University of Miami, School of Medicine, Miami, Florida, USA aut Novak Stana Department of Surgery, University of Miami, School of Medicine, Miami, Florida, USA aut Hsu Li-Chien Bentley Laboratories, Irvine, California, USA aut Perryman Richard Department of Surgery, University of Miami, School of Medicine, Miami, Florida, USA aut Serafini Aldo Department of Radiology, University of Miami, School of Medicine, Miami, Florida, USA aut Sfakianakis George Department of Radiology, University of Miami, School of Medicine, Miami, Florida, USA aut Duncan Robert Department of Epidemiology, University of Miami, School of Medicine, Miami, Florida, USA aut Dietrich W. Department of Neurology, University of Miami, School of Medicine, Miami, Florida, USA aut Horton Allan Coulter Corporation, Miami, Florida, USA aut Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/4(1996-12-01), 343-360 0929-5305 3:4<343 1996 3 11239 https://doi.org/10.1007/BF00133078 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00133078 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dewanjee Mrinal Department of Radiology, University of Miami, School of Medicine, Miami, Florida, USA aut NATIONALLICENCE 700 1- Wu Shu Department of Radiology, University of Miami, School of Medicine, Miami, Florida, USA aut NATIONALLICENCE 700 1- Kapadvanjwala Mansoor Department of Biomedical Engineering, University of Miami, School of Medicine, Miami, Florida, USA aut NATIONALLICENCE 700 1- De Dibyendu Department of Biomedical Engineering, University of Miami, School of Medicine, Miami, Florida, USA aut NATIONALLICENCE 700 1- Dewanjee Sumit Department of Radiology, University of Miami, School of Medicine, Miami, Florida, USA aut NATIONALLICENCE 700 1- Novak Stana Department of Surgery, University of Miami, School of Medicine, Miami, Florida, USA aut NATIONALLICENCE 700 1- Hsu Li-Chien Bentley Laboratories, Irvine, California, USA aut NATIONALLICENCE 700 1- Perryman Richard Department of Surgery, University of Miami, School of Medicine, Miami, Florida, USA aut NATIONALLICENCE 700 1- Serafini Aldo Department of Radiology, University of Miami, School of Medicine, Miami, Florida, USA aut NATIONALLICENCE 700 1- Sfakianakis George Department of Radiology, University of Miami, School of Medicine, Miami, Florida, USA aut NATIONALLICENCE 700 1- Duncan Robert Department of Epidemiology, University of Miami, School of Medicine, Miami, Florida, USA aut NATIONALLICENCE 700 1- Dietrich W. Department of Neurology, University of Miami, School of Medicine, Miami, Florida, USA aut NATIONALLICENCE 700 1- Horton Allan Coulter Corporation, Miami, Florida, USA aut NATIONALLICENCE 773 0- Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/4(1996-12-01), 343-360 0929-5305 3:4<343 1996 3 11239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer