caa a22 4500 477062075 CHVBK 20180405111403.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00133077 doi (NATIONALLICENCE)springer-10.1007/BF00133077 A simple, reproducible animal model of arterial occlusion with mixed thrombus [Elektronische Daten] [Shaojun Wang, Daniel Garner, Matthew Romano, Shi-Kaung Peng, Steven Goldberg, William French] Background: Current animal models of thrombotic arterial occlusion are complex and difficult to perform. A more simplified model of thrombotic occlusion with angiographic endpoints and mixed thrombotic composition would be useful to assess the effectiveness of new therapeutic modalities. Methods and results: Femoral arteries in 38 New Zealand White rabbits were cannulated. The animal protocol involved denudation of one iliac artery by stripping with progressively increasing balloon size, followed by inflow arterial occlusion and the addition of thrombin. Cineangiography was performed at baseline and 10 minutes after the procedure to assess TIMI blood flow. Baseline angiography revealed normal TIMI 3 flow in all arteries. A successful angiographic endpoint of TIMI 0 or TIMI 1 blood flow was achieved in only 6 or 46% of 13 rabbits with initial use of the protocol, but was successful in 25 or 100% of a consecutive series of rabbits after the initial or repeat use of the protocol. Importantly, histologic analysis of thrombus revealed a mixture of platelets, erythrocytes, and fibrin. The mean injury index of the internal elastic lamina (IEL) was 40.7 ± 35.16%. No correlation was found between TIMI flow change and intact IEL (R = \s-0.068, p > 0.5). Total procedure time was about 1 hour. Conclusion: This simple and reproducible animal model of arterial occlusion provided a mixed thrombus that is comparable to human thrombus and thus may be useful to assess thrombolytic agents or new coronary interventional devices. Kluwer Academic Publishers, 1996 thrombus nationallicence animal model nationallicence arterial occlusion nationallicence Wang Shaojun St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut Garner Daniel St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut Romano Matthew St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut Peng Shi-Kaung St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut Goldberg Steven St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut French William St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/4(1996-12-01), 337-341 0929-5305 3:4<337 1996 3 11239 https://doi.org/10.1007/BF00133077 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00133077 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Shaojun St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut NATIONALLICENCE 700 1- Garner Daniel St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut NATIONALLICENCE 700 1- Romano Matthew St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut NATIONALLICENCE 700 1- Peng Shi-Kaung St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut NATIONALLICENCE 700 1- Goldberg Steven St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut NATIONALLICENCE 700 1- French William St. John's Cardiovascular Research Center, Harbor-UCLA Medical Center, Torrance, California, USA aut NATIONALLICENCE 773 0- Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/4(1996-12-01), 337-341 0929-5305 3:4<337 1996 3 11239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer