caa a22 4500 477062083 CHVBK 20180405111403.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00133079 doi (NATIONALLICENCE)springer-10.1007/BF00133079 Ultrastructural evaluation of postischemic cell death (lethal reperfusion injury) in porcine hearts [Elektronische Daten] [Hermann Klein, Sibylle Pich, Stefanie Lindert-Heimberg, Klaus Nebendahl, Udo Sprengel, Hans-Helge M/:uller, Jutta Schaper] This study investigated whether reperfusion results in an increase of ultrastructurally determined myocardial injury in pig hearts. The left anterior descending coronary artery (LAD) was distally occluded in 12 pigs for 35-45 minutes and then reperfused for 3 hours. At the end of ischemia, as well as after 3 hours of reperfusion, one transmural biopsy was removed from the center of the risk region and subdivided into four specimens, representing the subendocardial (I), subendo-midmyocardial (II), subepi-midmyocardial (III), and subepicardial layers (IV). The degree of injury was assessed by electronmicroscopy and was scored as reversible (1), an almost equal mixture of reversible and irreversible (2), and totally irreversible (3) damage. In addition, infarct size was determined as the ratio of infarcted (tetrazolium stain) to ischemic (dye technique) myocardium. Infarct sizes ranged from 29.3% to 93% (mean 61.2%). The scores of injury of the four tissue layers before and after reperfusion did not differ significantly: layer I, 2.4 ± 0.8/2.3 ± 0.9; layer II, 2.2 ± 0.9/2.0 ± 0.9; layer III, 1.8 ± 0.9/2.0 ± 0.9; and layer IV, 1.6 ± 0.9/1.3 ± 0.6. The means of the four layers were almost identical at the end of ischemia (2.1 ± 0.8) and after 3 hours of reperfusion (2.0 ± 0.6). A linear regression analysis with 95% confidence limits of the score values before and after reperfusion indicated that maximally 25% of a mean final infarct size of about 50% may be due to lethal reperfusion injury. This study suggests that cell death in regional ischemia and reperfusion occurs predominantly during ischemia and not during reperfusion. Kluwer Academic Publishers, 1996 ischemia nationallicence infarction nationallicence infarct size nationallicence cell death nationallicence Klein Hermann Department of Cardiology, University of Marburg, Marburg, FRG aut Pich Sibylle Department of Cardiology, University of Marburg, Marburg, FRG aut Lindert-Heimberg Stefanie Department of Experimental Animal Research, University of Göttingen, Göttingen, FRG aut Nebendahl Klaus Department of Experimental Animal Research, University of Göttingen, Göttingen, FRG aut Sprengel Udo Max-Planck-Institute of Experimental Cardiology, Bad Nauheim, FRG aut M/:uller Hans-Helge Department of Medical Biometry, University of Marburg, Marburg, FRG aut Schaper Jutta Max-Planck-Institute of Experimental Cardiology, Bad Nauheim, FRG aut Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/4(1996-12-01), 361-366 0929-5305 3:4<361 1996 3 11239 https://doi.org/10.1007/BF00133079 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00133079 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Klein Hermann Department of Cardiology, University of Marburg, Marburg, FRG aut NATIONALLICENCE 700 1- Pich Sibylle Department of Cardiology, University of Marburg, Marburg, FRG aut NATIONALLICENCE 700 1- Lindert-Heimberg Stefanie Department of Experimental Animal Research, University of Göttingen, Göttingen, FRG aut NATIONALLICENCE 700 1- Nebendahl Klaus Department of Experimental Animal Research, University of Göttingen, Göttingen, FRG aut NATIONALLICENCE 700 1- Sprengel Udo Max-Planck-Institute of Experimental Cardiology, Bad Nauheim, FRG aut NATIONALLICENCE 700 1- M/:uller Hans-Helge Department of Medical Biometry, University of Marburg, Marburg, FRG aut NATIONALLICENCE 700 1- Schaper Jutta Max-Planck-Institute of Experimental Cardiology, Bad Nauheim, FRG aut NATIONALLICENCE 773 0- Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 3/4(1996-12-01), 361-366 0929-5305 3:4<361 1996 3 11239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer